PUBLICATION
Functional Development of the Zebrafish Pineal Gland: Light-Induced Expression of Period2 is Required for Onset of the Circadian Clock
- Authors
- Ziv, L., Levkovitz, S., Toyama, R., Falcon, J., and Gothilf, Y.
- ID
- ZDB-PUB-050510-5
- Date
- 2005
- Source
- Journal of neuroendocrinology 17(5): 314-320 (Journal)
- Registered Authors
- Falcon, Jack, Gothilf, Yoav, Toyama, Reiko
- Keywords
- none
- MeSH Terms
-
- Animals
- Arylalkylamine N-Acetyltransferase/genetics
- Arylalkylamine N-Acetyltransferase/metabolism*
- Arylalkylamine N-Acetyltransferase/radiation effects
- Biomarkers/metabolism
- Circadian Rhythm/genetics
- Circadian Rhythm/physiology*
- Circadian Rhythm/radiation effects
- Eye Proteins/genetics
- Eye Proteins/metabolism*
- Eye Proteins/radiation effects
- Female
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Fish Proteins/radiation effects
- Gene Expression Regulation, Developmental/radiation effects*
- Light
- Male
- Period Circadian Proteins
- Pineal Gland/embryology
- Pineal Gland/enzymology*
- Pineal Gland/radiation effects
- RNA, Messenger/analysis
- Zebrafish/embryology
- Zebrafish/metabolism*
- Zebrafish Proteins
- PubMed
- 15869567 Full text @ J. Neuroendocrinol.
Citation
Ziv, L., Levkovitz, S., Toyama, R., Falcon, J., and Gothilf, Y. (2005) Functional Development of the Zebrafish Pineal Gland: Light-Induced Expression of Period2 is Required for Onset of the Circadian Clock. Journal of neuroendocrinology. 17(5):314-320.
Abstract
Abstract In zebrafish, the pineal gland is a photoreceptive organ that contains an intrinsic circadian oscillator and exhibits rhythmic arylalkylamine-N-acetyltransferase (zfaanat2) mRNA expression. In the present study, we investigated the role of light and of a clock gene, zperiod2 (zper2), in the development of this rhythm. Analysis of zfaanat2 mRNA expression in the pineal gland of 3-day-old zebrafish embryos after exposure to different photoperiodic regimes indicated that light is required for proper development of the circadian clock-controlled rhythmic expression of zfaanat2, and that a 1-h light pulse is sufficient to initiate this rhythm. Analysis of zper2 mRNA expression in zebrafish embryos exposed to different photoperiodic regimes indicated that zper2 expression is transiently up-regulated by light but is not regulated by the circadian oscillator. To establish the association between light-induced zper2 expression and light-induced clock-controlled zfaanat2 rhythm, zPer2 knock-down experiments were performed. The zfaanat2 mRNA rhythm, induced by a 1-h light pulse, was abolished in zPer2 knock-down embryos. These experiments indicated that light-induced zper2 expression is crucial for establishment of the clock-controlled zfaanat2 rhythm in the zebrafish pineal gland.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping