PUBLICATION

Chemokine signaling guides axons within the retina in zebrafish

Authors
Li, Q., Shirabe, K., Thisse, C., Thisse, B., Okamoto, H., Masai, I., and Kuwada, J.Y.
ID
ZDB-PUB-050221-5
Date
2005
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   25(7): 1711-1717 (Journal)
Registered Authors
Kuwada, John, Li, Qin, Masai, Ichiro, Okamoto, Hitoshi, Thisse, Bernard, Thisse, Christine
Keywords
zebrafish; retinal ganglion cell; SDF-1; CXCR4; axonogenesis; axon
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Chemokine CXCL12
  • Chemokines, CXC/genetics
  • Chemokines, CXC/physiology*
  • Gene Targeting
  • Growth Cones/physiology*
  • Growth Cones/ultrastructure
  • Mosaicism
  • Oligodeoxyribonucleotides, Antisense/pharmacology
  • Optic Nerve/cytology*
  • Optic Nerve/embryology
  • Phenotype
  • Receptors, CXCR4/genetics
  • Receptors, CXCR4/physiology*
  • Recombinant Fusion Proteins/physiology
  • Retina/embryology
  • Retina/ultrastructure*
  • Retinal Ganglion Cells/cytology*
  • Signal Transduction/physiology*
  • Zebrafish/embryology
PubMed
15716407 Full text @ J. Neurosci.
Abstract
Chemokines are a large family of secreted proteins that play an important role in the migration of leukocytes during hematopoiesis and inflammation. Chemokines and their receptors are also widely distributed in the CNS. Although recent investigations are beginning to elucidate chemokine function within the CNS, relatively little is known about the CNS function of this important class of molecules. To better appreciate the CNS function of chemokines, the role of signaling by stromal cell-derived factor-1 (SDF-1) through its receptor, chemokine (CXC motif) receptor 4 (CXCR4), was analyzed in zebrafish embryos. The SDF-1/CXCR4 expression pattern suggested that SDF-1/CXCR4 signaling was important for guiding retinal ganglion cell axons within the retina to the optic stalk to exit the retina. Antisense knockdown of the ligand and/or receptor and a genetic CXCR4 mutation both induced retinal axons to follow aberrant pathways within the retina. Furthermore, retinal axons deviated from their normal pathway and extended to cells ectopically expressing SDF-1 within the retina. These data suggest that chemokine signaling is both necessary and sufficient for directing retinal growth cones within the retina.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes