PUBLICATION
Scl is required for dorsal aorta as well as blood formation in zebrafish embryos
- Authors
- Patterson, L.J., Gering, M., and Patient, R.
- ID
- ZDB-PUB-050113-4
- Date
- 2005
- Source
- Blood 105(9): 3502-3511 (Journal)
- Registered Authors
- Gering, Martin, Patient, Roger K., Patterson, Lucy
- Keywords
- none
- MeSH Terms
-
- Animals
- Aorta/embryology
- Aorta/growth & development*
- Basic Helix-Loop-Helix Transcription Factors
- Blood*
- Blood Circulation/genetics
- DNA-Binding Proteins/deficiency
- DNA-Binding Proteins/physiology*
- Embryo, Nonmammalian
- Endothelium, Vascular/cytology
- Endothelium, Vascular/embryology
- Endothelium, Vascular/growth & development
- Gene Expression Regulation, Developmental
- Hematopoietic Stem Cells/cytology
- Mice
- Neovascularization, Physiologic
- Proto-Oncogene Proteins/deficiency
- Proto-Oncogene Proteins/physiology*
- Transcription Factors/deficiency
- Transcription Factors/physiology*
- Zebrafish
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/physiology*
- PubMed
- 15644413 Full text @ Blood
Citation
Patterson, L.J., Gering, M., and Patient, R. (2005) Scl is required for dorsal aorta as well as blood formation in zebrafish embryos. Blood. 105(9):3502-3511.
Abstract
Blood and endothelial cells arise in close association in developing embryos, possibly from a shared precursor, the haemangioblast, or as haemogenic endothelium. The transcription factor, Scl/Tal1, is essential for haematopoiesis but thought to be required only for remodelling of endothelium in mouse embryos. By contrast, it has been implicated in haemangioblast formation in embryoid bodies. To resolve the role of scl in endothelial development, we knocked down its synthesis in zebrafish embryos where early precursors and later phenotypes can be more easily monitored. With respect to blood, the zebrafish morphants phenocopied the mouse knockout and positioned scl in the genetic hierarchy. Importantly, endothelial development was also clearly disrupted. Dorsal aorta formation was substantially compromised and gene expression in the posterior cardinal vein was abnormal. We conclude that scl is especially critical for the development of arteries where adult haematopoietic stem cells emerge, implicating scl in the formation of haemogenic endothelium.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping