PUBLICATION
Contrast sensitivity, spatial and temporal tuning of the larval zebrafish optokinetic response
- Authors
- Rinner, O., Rick, J.M., and Neuhauss, S.C.
- ID
- ZDB-PUB-050104-7
- Date
- 2005
- Source
- Investigative ophthalmology & visual science 46(1): 137-142 (Journal)
- Registered Authors
- Neuhauss, Stephan, Rick, Jens, Rinner, Oliver
- Keywords
- none
- MeSH Terms
-
- Animals
- Behavior, Animal/physiology
- Contrast Sensitivity/physiology*
- Larva/physiology
- Light
- Nystagmus, Optokinetic/physiology*
- Space Perception/physiology*
- Zebrafish/physiology*
- PubMed
- 15623766 Full text @ Invest. Ophthalmol. Vis. Sci.
Citation
Rinner, O., Rick, J.M., and Neuhauss, S.C. (2005) Contrast sensitivity, spatial and temporal tuning of the larval zebrafish optokinetic response. Investigative ophthalmology & visual science. 46(1):137-142.
Abstract
purpose. To characterize the quantitative properties of the optokinetic response (OKR) in zebrafish larvae as a tool to test visual performance in genetically modified larvae. methods. Horizontal OKR was triggered in 5-day-old zebrafish larvae by stimulation with projected computer-generated gratings of varying contrast, angular velocity, temporal and spatial frequency, and brightness. Eye movements were analyzed by a custom-made eye tracker based on image analysis. results. The gain of the OKR slow phase was dependent on angular velocity, spatial frequency, and contrast of a moving grating, but largely independent on brightness. Eye velocity was a logarithmically linear function of grating contrast with a slope of approximately 0.8 per log unit contrast. conclusions. The OKR of the larval zebrafish is not scaled for stimulus contrast and spatial frequency. These properties make the OKR a valuable tool to quantify behavioral visual performance such as visual acuity, contrast sensitivity, and light adaptation. This behavioral paradigm will be useful for analyzing visual performance in mutant and gene-knockdown larval zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping