ZFIN ID: ZDB-PUB-050104-3
Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish
Rhodes, J., Hagen, A., Hsu, K., Deng, M., Liu, T.X., Look, A.T., and Kanki, J.P.
Date: 2005
Source: Developmental Cell   8(1): 97-108 (Journal)
Registered Authors: Hsu, Karl, Kanki, John, Liu, Ting Xi, Look, A. Thomas, Rhodes, Jennifer
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/physiology
  • Cell Movement/physiology
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology*
  • Embryonic Induction
  • Erythroid Precursor Cells/physiology*
  • Erythroid-Specific DNA-Binding Factors
  • Flow Cytometry/methods
  • GATA1 Transcription Factor
  • Gene Expression Regulation, Developmental/physiology
  • Genotype
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Hematopoiesis/physiology
  • In Situ Hybridization/methods
  • Microinjections/methods
  • Models, Biological
  • Myeloid Progenitor Cells/physiology*
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology*
  • RNA, Messenger/biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Trans-Activators/genetics
  • Trans-Activators/physiology*
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Transplantation/methods
  • Zebrafish/embryology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 15621533 Full text @ Dev. Cell
The zebrafish is a powerful model system for investigating embryonic vertebrate hematopoiesis, allowing for the critical in vivo analysis of cell lineage determination. In this study, we identify zebrafish myeloerythroid progenitor cells (MPCs) that are likely to represent the functional equivalent of mammalian common myeloid progenitors. Utilizing transgenic pu.1-GFP fish, real-time MPC differentiation was correlated with dynamic changes in cell motility, morphology, and gene expression. Unlike mammalian hematopoiesis, embryonic zebrafish myelopoiesis and erythropoiesis occur in anatomically separate locations. Gene knockdown experiments and transplantation assays demonstrated the reciprocal negative regulation of pu.1 and gata1 and their non-cell-autonomous regulation that determines myeloid versus erythroid MPC fate in the distinct blood-forming regions. Furthermore, forced expression of pu.1 in the bloodless mutant cloche resulted in myelopoietic rescue, providing intriguing evidence that this gene can function in the absence of some stem cell genes, such as scl, in governing myelopoiesis.