PUBLICATION

Expression of a novel zebrafish zinc finger gene, gli2b, is affected in Hedgehog and Notch signaling related mutants during embryonic development

Authors
Ke, Z., Emelyanov, A., Lim, S.E., Korzh, V., and Gong, Z.
ID
ZDB-PUB-041228-13
Date
2005
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   232(2): 479-486 (Journal)
Registered Authors
Gong, Zhiyuan, Ke, Zhiyuan, Korzh, Vladimir
Keywords
hindbrain, rhombomere, hypothalamus, sonic you, smoothened, you too, mindbomb, zinc finger, gene duplication, ortholog
MeSH Terms
  • DNA, Complementary/metabolism
  • Phosphorylation
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics*
  • Models, Genetic
  • Zebrafish
  • Reverse Transcriptase Polymerase Chain Reaction
  • Trans-Activators/biosynthesis*
  • Trans-Activators/genetics
  • In Situ Hybridization
  • Immunohistochemistry
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Mutation*
  • Time Factors
  • Protein Structure, Tertiary
  • Chromosome Mapping
  • Transcription Factors/biosynthesis*
  • Transcription Factors/genetics*
  • Zinc Fingers
  • Hedgehog Proteins
  • Phylogeny
  • Animals
  • RNA, Messenger/metabolism
  • Signal Transduction
  • Amino Acid Sequence
(all 27)
PubMed
15614761 Full text @ Dev. Dyn.
Abstract
Gli zinc-finger proteins are known as downstream mediators of the evolutionary conserved Hedgehog pathway. In zebrafish, gli2 functions differently from Gli2 in mammals. This difference could be due to the gli2 duplication in teleosts evolution and partial redundancy between two duplicated genes. Here, we report a novel zebrafish gli2-like cDNA. Its structure, genetic location, and distinct expression pattern in the central nervous system suggested that this gene might represent a second gli2 of teleosts, and we named it gli2b. gli2b was expressed in the neural keel, excluding the forebrain-midbrain boundary, while gli2 expression complemented this pattern. After 24 hours postfertilization, several specific domains of gli2b expression were observed in the lateral and medial hindbrain and hypothalamus. In mutants affecting the Hedgehog and Notch signaling pathways, gli2b expression was either disrupted or extended in different regions. Developmental Dynamics 232:480-487, 2005. (c) 2005 Wiley-Liss, Inc.
Genes / Markers
Figures
Figure Gallery (2 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mib1_unspecified
    		Unspecified
    shha_unspecified
      		Unspecified
      smo_unspecified
        		Unspecified
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        mib1unspecified
        shhaunspecified
        smounspecified
        WT
        1 - 4 of 4
        Show
        Antibodies
        No data available
        Orthology
        Gene Orthology
        gli2a
        gli2b
        1 - 2 of 2
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        Engineered Foreign Genes
        No data available
        Mapping
        Entity Type Entity Symbol Location
        GENEgli2bChr: 11 Details
        SSLPz10727Chr: 11 Details
        1 - 2 of 2
        Show
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        Citation
        Ke, Z., Emelyanov, A., Lim, S.E., Korzh, V., and Gong, Z. (2005) Expression of a novel zebrafish zinc finger gene, gli2b, is affected in Hedgehog and Notch signaling related mutants during embryonic development. Developmental Dynamics : an official publication of the American Association of Anatomists. 232(2):479-486.