PUBLICATION
Characterization of snRNA and snRNA-type genes in the pufferfish Fugu rubripes
- Authors
- Myslinski, E., Krol, A., and Carbon, P.
- ID
- ZDB-PUB-041130-15
- Date
- 2004
- Source
- Gene 330: 149-158 (Journal)
- Registered Authors
- Krol, Alain
- Keywords
- Oct-1; Pol II; Pol III; tRNASec gene; Staf; zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites/genetics
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- DNA-Binding Proteins/metabolism
- Female
- Genome
- Host Cell Factor C1
- Molecular Sequence Data
- Octamer Transcription Factor-1
- Oocytes/metabolism
- Promoter Regions, Genetic/genetics
- Protein Binding
- RNA, Small Nuclear/genetics*
- Regulatory Sequences, Nucleic Acid/genetics
- Response Elements/genetics
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Takifugu/genetics*
- Transcription Factors/metabolism
- Xenopus Proteins
- Xenopus laevis
- PubMed
- 15087134 Full text @ Gene
Citation
Myslinski, E., Krol, A., and Carbon, P. (2004) Characterization of snRNA and snRNA-type genes in the pufferfish Fugu rubripes. Gene. 330:149-158.
Abstract
Vertebrate snRNA and snRNA-type genes occur in independent transcription units with external promoters. The transcription level from the basal promoter is enhanced by the distal sequence element DSE. This element contains almost invariably two activator submotifs, the Staf binding site and the octamer motif, recruiting the Staf and Oct-1 transcriptional activators. In the present work, database search identified 35 snRNA and snRNA-type genes in the genome sequence of the pufferfish Fugu rubripes. Sequence comparisons of promoter elements, determination of template activities by microinjection into Xenopus oocytes and DNA binding assays of the transcriptional activators led to the surprising finding that only two Fugu genes conform to the general scheme with the expected two submotifs in the DSE. Distinctively, all the other DSEs harbor a unique Staf binding site. Also striking was the observation that the tRNA(Sec), and the snRNA genes that are tandemly repeated, are transcribed from promoter-less DSEs. Evolutionary implications of these results are discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping