PUBLICATION

Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes

Authors

Waxman, J.S., Hocking, A.M., Stoick, C.L., and Moon, R.T.

ID

ZDB-PUB-041115-3

Date

2004

Source

Development (Cambridge, England) 131(23): 5909-5921 (Journal)

Registered Authors

Moon, Randall T., Waxman, Joshua

Keywords

Dishevelled, Dapper, Zebrafish, Wnt signaling

MeSH Terms

Cloning, Molecular
Immunohistochemistry
Cytoskeletal Proteins/metabolism
Signal Transduction
Phylogeny
Xenopus Proteins
Proteins/metabolism
Trans-Activators/metabolism
Nuclear Proteins/physiology*
Animals
Reverse Transcriptase Polymerase Chain Reaction
Cell Line
Humans
Gene Expression Regulation, Developmental*
Zebrafish
Intercellular Signaling Peptides and Proteins/metabolism*
Neoplasm Proteins/physiology*
Xenopus
Transfection
beta Catenin
Calcium/metabolism
Genome
Intracellular Signaling Peptides and Proteins/genetics*
Intracellular Signaling Peptides and Proteins/physiology*
Carrier Proteins/physiology*
Zebrafish Proteins/genetics*
Zebrafish Proteins/physiology*
Immunoprecipitation
Wnt Proteins
Adaptor Proteins, Signal Transducing

(all 30)

PubMed

15539487 Full text @ Development

Abstract

Wnt signaling pathways in vertebrates use the phosphoprotein Dishevelled (Dvl). The cellular responses to Wnt signaling may in part be modulated by Dvl-associated proteins, including Dapper (Dpr). We have cloned and characterized the zebrafish Dpr paralogs Dpr1 and Dpr2. Loss-of-function studies reveal that endogenous Dpr1 but not Dpr2 is required to enhance Wnt/beta-catenin activity in zebrafish embryos that are hypomorphic for Wnt8. Conversely, Dpr2 but not Dpr1 is required for normal convergence extension movements in embryos that are hypomorphic for Stbm or Wnt11, supporting a functional interaction of Dpr2 with Wnt/Ca(2+)-PCP signaling. In gain-of-function experiments, Dpr1 but not Dpr2 induces Wnt/beta-catenin target genes. Dpr1 synergizes with zebrafish Dvl2, and with the Dvl-interacting kinases CK1epsilon, Par1 and CK2, in activating target genes. We conclude that two Dvl-associated paralogs, Dpr1 and Dpr2, participate in distinct Wnt-dependent developmental processes.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
dact1	WT	standard conditions	75%-epiboly		ectoderm	ISH	Fig. 2
dact1	WT	standard conditions	Shield		margin	ISH	Fig. 2
dact1	WT	standard conditions	75%-epiboly		mesoderm	ISH	Fig. 2
dact1	WT	standard conditions	Bud		neural plate	ISH	Fig. 2
dact1	WT	standard conditions	5-9 somites		neural rod	ISH	Fig. 2
dact1	WT	standard conditions	5-9 somites	Not Detected	notochord	ISH	Fig. 2
dact1	WT	standard conditions	10-13 somites		paraxial mesoderm	ISH	Fig. 2
dact1	WT	standard conditions	Shield		shield	ISH	Fig. 2
dact1	WT	standard conditions	10-13 somites		somite	ISH	Fig. 2
dact1	WT	standard conditions	Bud		spinal cord neural plate	ISH	Fig. 2

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-dact1	standard conditions	10-13 somites	diencephalon decreased size, abnormal	Fig. 3
WT + MO1-dact1	standard conditions	10-13 somites	midbrain decreased size, abnormal	Fig. 3
WT + MO1-dact1	standard conditions	10-13 somites	whole organism decreased size, abnormal	Fig. 3
WT + MO1-dact1	standard conditions	Prim-5	telencephalon increased size, abnormal	Fig. 4
WT + MO1-dact1 + MO1-dact2	standard conditions	10-13 somites	convergent extension involved in axis elongation disrupted, abnormal	Fig. 3
WT + MO1-dact1 + MO1-dact2	standard conditions	10-13 somites	diencephalon decreased size, abnormal	Fig. 3
WT + MO1-dact1 + MO1-dact2	standard conditions	10-13 somites	midbrain decreased size, abnormal	Fig. 3
WT + MO1-dact1 + MO1-dact2	standard conditions	10-13 somites	notochord increased width, abnormal	Fig. 3
WT + MO1-dact1 + MO1-dact2	standard conditions	10-13 somites	somite increased width, abnormal	Fig. 3
WT + MO1-dact1 + MO1-dact2	standard conditions	10-13 somites	whole organism decreased length, abnormal	Fig. 3

1 - 10 of 39

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dact1	MO1-dact1	MRPHLNO
dact1	MO2-dact1	MRPHLNO
dact2	MO1-dact2	MRPHLNO
dact2	MO2-dact2	MRPHLNO
vangl2	MO1-vangl2	MRPHLNO
wnt8a	MO1-wnt8a	MRPHLNO
wnt8a	MO2-wnt8a	MRPHLNO
wnt11f2	MO1-wnt11f2	MRPHLNO

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Fish

Fish
WT
WT + MO1-dact1
WT + MO1-dact1 + MO1-dact2
WT + MO1-dact1 + MO1-wnt8a + MO2-wnt8a
WT + MO1-dact2
WT + MO1-dact2 + MO1-vangl2
WT + MO1-dact2 + MO1-wnt11f2
WT + MO1-vangl2
WT + MO1-wnt8a + MO2-wnt8a
WT + MO1-wnt11f2

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Antibodies

Orthology

Gene	Orthology
dact2

Engineered Foreign Genes

Mapping

Waxman et al., 2004 ZDB-PUB-041115-3 PMID:15539487

Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes

Waxman et al., 2004

ZDB-PUB-041115-3
PMID:15539487