ZFIN ID: ZDB-PUB-041111-8
Induction of reversible hemolytic anemia in living zebrafish using a novel small molecule
Shafizadeh, E., Peterson, R.T., and Lin, S.
Date: 2004
Source: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   138(3): 245-249 (Journal)
Registered Authors: Lin, Shuo, Peterson, Randall, Shafizadeh, Ebrahim
Keywords: Zebrafish; Small molecules; Hemolytic anemia; Hematopoiesis; Phenylhydrazine; Heinz bodies; GATA1; Transgenic zebrafish
MeSH Terms:
  • Anemia, Hemolytic/chemically induced*
  • Anemia, Hemolytic/drug therapy
  • Anemia, Hemolytic/pathology*
  • Animals
  • Disease Models, Animal
  • Drug Evaluation, Preclinical/methods*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/pathology
  • Erythroid Cells/drug effects
  • Erythroid Cells/pathology
  • Erythropoiesis/drug effects
  • Furans/chemistry
  • Furans/pharmacology*
  • Molecular Structure
  • Phenylhydrazines
  • Propionates/chemistry
  • Propionates/pharmacology*
  • Zebrafish/physiology*
PubMed: 15533782 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
We used zebrafish to screen and identify small molecules that affect the process of vertebrate hematopoietic development. Zebrafish embryos were exposed to a library of 5000 synthetic compounds and screened for defects in primitive erythropoiesis. Here, we present the characterization of hemolytic anemia induced in zebrafish by the small molecule 5115318 (3-[5-methyl-furan 2-yl]-propionic acid N'-phenyl-hydrazide). This compound is capable of generating hemoglobin aggregates and Heinz bodies in red cells in vivo only. The induced anemia is reversible and treated fish recover in about 4 days. This study shows the feasibility of using zebrafish to screen for small molecules that can modulate the specific process of erythropoiesis.