The Cellular Retinol-Binding Protein Genes are Duplicated and Differentially Transcribed in the Developing and Adult Zebrafish (Danio rerio)

Liu, R.Z., Sun, Q., Thisse, C., Thisse, B., Wright, J.M., and Denovan-Wright, E.M.
Mol. Biol. Evol.   22(3): 469-477 (Journal)
Registered Authors
Thisse, Bernard, Thisse, Christine, Wright, Jonathan M.
gene duplication, gene structure, phylogeny, genome evolution, gene expression, subfunctionalization
MeSH Terms
  • Animals
  • Female
  • Gene Duplication*
  • Gene Expression Regulation, Developmental*/physiology
  • Humans
  • Male
  • Mice
  • Organ Specificity/genetics
  • Organ Specificity/physiology
  • Rats
  • Retinol-Binding Proteins/genetics*
  • Retinol-Binding Proteins, Cellular
  • Transcription, Genetic/physiology
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
15509725 Full text @ Mol. Biol. Evol.
There are single copies of the genes encoding the cellular retinol-binding protein type I and II (CRBPI and CRBPII) in the human and rodent genomes. We have identified duplicate genes for both CRBPI and CRBPII in the zebrafish (Danio rerio) genome (rbp1b and rbp2b). The zebrafish rbp1b and rbp2b have conserved gene structures, amino acid sequence similarities, gene phylogenies and syntenic relationships with their mammalian orthologs and zebrafish paralogs, rbp1a and rbp2a. Like the mammalian genes for CRBPI and CRBPII, the zebrafish rbp1b and rbp2b genes are closely linked on a single linkage group. Comparative analysis suggests that the duplicate genes of rbp1 and rbp2 in the zebrafish genome may have arisen by chromosomal or whole genome duplication. During embryonic development, rbp1b transcripts were detected in the gall bladder of 5 day post-fertilization (dpf) larvae. The rbp2b mRNA was abundant in the developing liver through 48 hours post-fertilization (hpf) to 5 dpf. Using reverse transcriptionpolymerase chain reaction (RT-PCR), rbp1b transcripts were detected in the ovary while rbp2b mRNA was observed predominantly in the adult liver. Tissue section in situ hybridization and emulsion autoradiography localized rbp1b mRNA to primary oocytes within the zebrafish ovary. The differential mRNA distribution patterns of the rbp1a, rbp1b, rbp2a and rbp2b genes in the developing and adult zebrafish suggest that shuffling of subfunctions among duplicate copies of paralogous genes may be a mechanism for the retention of duplicated genes in vertebrates.
Genes / Markers
Show all Figures
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes