PUBLICATION
Analysis of early epidermal development in zebrafish
- Authors
- Webb, A.E., and Kimelman, D.
- ID
- ZDB-PUB-041028-3
- Date
- 2005
- Source
- Methods in molecular biology (Clifton, N.J.) 289: 137-146 (Chapter)
- Registered Authors
- Kimelman, David, Webb, Ashley
- Keywords
- Epidermis; zebrafish; in situ hybridization; bromodeoxyuridine; TUNEL; immunocytochemistry
- MeSH Terms
-
- Animals
- Cell Proliferation*
- Embryo, Nonmammalian/cytology
- Epidermis/cytology*
- Gene Expression Regulation, Developmental/genetics*
- Immunohistochemistry
- In Situ Hybridization
- Zebrafish/anatomy & histology*
- Zebrafish/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 15502179 Full text @ Meth. Mol. Biol.
Citation
Webb, A.E., and Kimelman, D. (2005) Analysis of early epidermal development in zebrafish. Methods in molecular biology (Clifton, N.J.). 289:137-146.
Abstract
The zebrafish (Danio rerio) is a member of the teleost (bony fish) lineage that diverged from the mammalian lineage 420 million years ago. Despite this ancient divergence, there are important similarities between the epidermis of fish and mammals. In addition, recent work suggests that the genes required for epidermal development in the zebrafish also are essential for this process in higher vertebrates, indicating that analysis of the zebrafish system will contribute to understanding mouse and human development. The zebrafish is a very useful genetic system, and many mutants with epidermal defects have been obtained in large-scale genetic screens. In addition, zebrafish embryos are transparent, develop externally, and can be collected in abundance. The aim of this chapter is to provide the researcher with basic protocols to examine gene expression, cell proliferation, and cell death in the developing zebrafish. Although only a few specific epidermal markers have been identified in the zebrafish thus far, we also provide a list of useful genes for epidermal analysis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping