PUBLICATION
Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos
- Authors
- Herbomel, P., and Levraud, J.P.
- ID
- ZDB-PUB-041022-2
- Date
- 2005
- Source
- Methods in molecular medicine 105: 199-214 (Chapter)
- Registered Authors
- Herbomel, Philippe, Levraud, Jean-Pierre
- Keywords
- Zebrafish; macrophages; DIC microscopy; Nomarski; video microscopy; embryo; hematopoiesis
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology*
- Cell Movement/physiology*
- Macrophages/physiology*
- Microscopy, Confocal/methods*
- Microscopy, Video/methods*
- Zebrafish/embryology*
- PubMed
- 15492397 Full text @ Methods Mol. Med.
Citation
Herbomel, P., and Levraud, J.P. (2005) Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos. Methods in molecular medicine. 105:199-214.
Abstract
Because zebrafish embryos are transparent, cell behaviors and interactions can be directly imaged noninvasely in live embryos using differential interference contrast-Nomarski light microscopy. We found that the imaging quality can be much improved by coupling differential interference contrast-Nomarski to true (analogical) color video so as to visualize the image in real time on a high-resolution colour video monitor. We explain here how to apply this approach to the in vivo imaging of embryonic macrophages, which constitute a distinct early macrophage lineage, that originates from the rostral-most lateral mesoderm -adjacent to the cardiac field, differentiate in the yolk sac, and rapidly spread in embryonic tissues, although still retaining proliferative capacity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping