ZFIN ID: ZDB-PUB-040920-7
Screen for genes differentially expressed during regeneration of the zebrafish caudal fin
Padhi, B.K., Joly, L., Tellis, P., Smith, A., Nanjappa, P., Chevrette, M., Ekker, M., and Akimenko, M.A.
Date: 2004
Source: Developmental dynamics : an official publication of the American Association of Anatomists   231(3): 527-541 (Journal)
Registered Authors: Akimenko, Marie-Andree, Chevrette, Mario, Ekker, Marc, Joly, Lucille, Padhi, Bhaja, Tellis, Patricia
Keywords: suppression subtractive hybridization (SSH), differential display reverse transcriptase polymerase chain reaction (DDRT-PCR), in situ hybridization, gene mapping, epimorphic regeneration, fin development, wound healing, blastema, lepidotrichia, dermal bone, keratin
MeSH Terms:
  • Amputation
  • Animals
  • Base Sequence
  • DNA, Complementary/chemistry
  • DNA, Complementary/genetics
  • DNA, Complementary/isolation & purification
  • Embryo, Nonmammalian
  • Expressed Sequence Tags
  • Extremities/embryology*
  • Extremities/physiology
  • Gene Expression Regulation, Developmental*
  • Genetic Testing*
  • In Situ Hybridization
  • Keratins/genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization/methods
  • Physical Chromosome Mapping
  • Polymerase Chain Reaction
  • Regeneration/genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation
  • Wound Healing/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology
PubMed: 15376328 Full text @ Dev. Dyn.
The zebrafish caudal fin constitutes an important model for studying the molecular basis of tissue regeneration. The cascade of genes induced after amputation or injury, leading to restoration of the lost fin structures, include those responsible for wound healing, blastema formation, tissue outgrowth, and patterning. We carried out a systematic study to identify genes that are up-regulated during initiation (1 day) and outgrowth and differentiation (4 days) of fin regeneration by using two complementary methods, suppression subtraction hybridization (SSH) and differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). We obtained 298 distinct genes/sequences from SSH libraries and 24 distinct genes/sequences by DDRT-PCR. We determined the expression of 54 of these genes using in situ hybridization. In parallel, gene expression analyses were done in zebrafish embryos and early larvae. The information gathered from the present study provides resources for further investigations into the molecular mechanisms of fin development and regeneration.