PUBLICATION
Organization and function of microfilaments during late epiboly in zebrafish embryos
- Authors
- Cheng, J.C., Miller, A.L., and Webb, S.E.
- ID
- ZDB-PUB-040916-3
- Date
- 2004
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 231(2): 313-323 (Journal)
- Registered Authors
- Cheng, Chong Nam, Miller, Andrew L., Webb, Sarah E.
- Keywords
- zebrafish, epiboly, microfilaments, endocytosis
- MeSH Terms
-
- Actin Cytoskeleton/metabolism*
- Actins/metabolism
- Animals
- Cytochalasin B/pharmacology
- Cytoskeleton/metabolism
- Egtazic Acid/analogs & derivatives
- Egtazic Acid/chemistry
- Egtazic Acid/pharmacology
- Embryo, Nonmammalian*/anatomy & histology
- Embryo, Nonmammalian*/drug effects
- Embryo, Nonmammalian*/physiology
- Morphogenesis*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 15366008 Full text @ Dev. Dyn.
Citation
Cheng, J.C., Miller, A.L., and Webb, S.E. (2004) Organization and function of microfilaments during late epiboly in zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 231(2):313-323.
Abstract
We report that, during epiboly in zebrafish, three F-actin-based structures appear only after the blastoderm migrates past the embryonic equator. They are composed of two ring-like F-actin structures that form at the deep cell and enveloping layer margins of the blastoderm and a punctate actin band that develops in the external yolk syncytial layer. Treatment with cytochalasin B or the calcium chelator dibromo-BAPTA results in the disruption of all three of these actin-based structures, leading to the slowing or immediate arrest of epiboly, respectively, followed by a failure of yolk cell occlusion and the eventual lysis of the embryo through the vegetal pole region. We suggest, therefore, that these structures function in the occlusion of the vegetal portion of the yolk cell during the latter stages of epiboly. Possible roles for these new structures, their modulation by Ca(2+), as well as the functions of other previously described F-actin-based structures observed throughout epiboly, are discussed. Developmental Dynamics 231:313-323, 2004.Copyright 2004 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping