PUBLICATION
A Crucial Interaction between Embryonic Red Blood Cell Progenitors and Paraxial Mesoderm Revealed in spadetail Embryos
- Authors
- Rohde, L.A., Oates, A.C., and Ho, R.K.
- ID
- ZDB-PUB-040810-5
- Date
- 2004
- Source
- Developmental Cell 7(2): 251-262 (Journal)
- Registered Authors
- Ho, Robert K., Oates, Andrew, Rohde, Laurel
- Keywords
- none
- MeSH Terms
-
- Embryo, Nonmammalian/metabolism*
- In Situ Hybridization
- Mesoderm/metabolism*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Time Factors
- Animals
- Cell Transplantation
- Erythrocytes/cytology*
- Erythrocytes/metabolism*
- Models, Biological
- Gene Expression Regulation
- Embryo, Mammalian
- Stem Cells
- T-Box Domain Proteins/genetics*
- Microcirculation
- Protein Binding
- Gene Expression Regulation, Developmental*
- Image Processing, Computer-Assisted
- Zebrafish
- Mutation
- Microscopy, Fluorescence
- Hematopoietic Stem Cells/cytology
- PubMed
- 15296721 Full text @ Dev. Cell
Citation
Rohde, L.A., Oates, A.C., and Ho, R.K. (2004) A Crucial Interaction between Embryonic Red Blood Cell Progenitors and Paraxial Mesoderm Revealed in spadetail Embryos. Developmental Cell. 7(2):251-262.
Abstract
Zebrafish embryonic red blood cells (RBCs) develop in trunk intermediate mesoderm (IM), and early macrophages develop in the head, suggesting that local microenvironmental cues regulate differentiation of these two blood lineages. spadetail (spt) mutant embryos, which lack trunk paraxial mesoderm (PM) due to a cell-autonomous defect in tbx16, fail to produce embryonic RBCs but retain head macrophage development. In spt mutants, initial hematopoietic gene expression is absent in trunk IM, although endothelial and pronephric expression is retained, suggesting that early blood progenitor development is specifically disrupted. Using cell transplantation, we reveal that spt is required cell autonomously for early hematopoietic gene expression in trunk IM. Further, we uncover an interaction between embryonic trunk PM and blood progenitors that is essential for RBC development. Importantly, our data identify a hematopoietic microenvironment that allows embryonic RBC production in the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping