ZFIN ID: ZDB-PUB-040709-4
Progress towards cell-mediated gene transfer in zebrafish
Fan, L., and Collodi, P.
Date: 2002
Source: Briefings in functional genomics & proteomics   1(2): 131-138 (Journal)
Registered Authors: Collodi, Paul, Fan, Lianchun
Keywords: none
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/cytology
  • Gene Transfer Techniques*
  • Germ Cells
  • Stem Cells/cytology
  • Zebrafish/genetics*
PubMed: 15239899 Full text @ Brief. Funct. Genomic. Proteomic.
Although the zebrafish possesses several favourable characteristics that make it an ideal model for genetic studies of vertebrate development, one disadvantage of this model system is the absence of methods for the production of gene knockouts. The authors' laboratory, and others, are working to develop zebrafish pluripotent embryonic stem (ES) and primordial germ cell (PGC) cultures that can be used for cell-mediated gene transfer and the production of knockout mutant lines of fish. Progress has been made in developing short-term cell cultures that possess the ability to contribute to multiple tissues, including the germ line of a host embryo, and transgenic lines of zebrafish have been established using the embryo cell cultures. Work is in progress to extend the length of time that the embryo cells can be maintained in culture without losing their ability to generate germ-line chimeras.