PUBLICATION

Progress towards cell-mediated gene transfer in zebrafish

Authors
Fan, L., and Collodi, P.
ID
ZDB-PUB-040709-4
Date
2002
Source
Briefings in functional genomics & proteomics   1(2): 131-138 (Journal)
Registered Authors
Collodi, Paul, Fan, Lianchun
Keywords
none
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/cytology
  • Gene Transfer Techniques*
  • Germ Cells
  • Stem Cells/cytology
  • Zebrafish/genetics*
PubMed
15239899 Full text @ Brief. Funct. Genomic. Proteomic.
Abstract
Although the zebrafish possesses several favourable characteristics that make it an ideal model for genetic studies of vertebrate development, one disadvantage of this model system is the absence of methods for the production of gene knockouts. The authors' laboratory, and others, are working to develop zebrafish pluripotent embryonic stem (ES) and primordial germ cell (PGC) cultures that can be used for cell-mediated gene transfer and the production of knockout mutant lines of fish. Progress has been made in developing short-term cell cultures that possess the ability to contribute to multiple tissues, including the germ line of a host embryo, and transgenic lines of zebrafish have been established using the embryo cell cultures. Work is in progress to extend the length of time that the embryo cells can be maintained in culture without losing their ability to generate germ-line chimeras.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping