PUBLICATION

Roles for GFRalpha1 receptors in zebrafish enteric nervous system development

Authors
Shepherd, I.T., Pietsch, J., Elworthy, S., Kelsh, R.N., and Raible, D.W.
ID
ZDB-PUB-040628-1
Date
2004
Source
Development (Cambridge, England)   131(1): 241-249 (Journal)
Registered Authors
Elworthy, Stone, Kelsh, Robert, Raible, David, Shepherd, Iain T.
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Chickens
  • Conserved Sequence
  • Embryo, Nonmammalian/physiology
  • Glial Cell Line-Derived Neurotrophic Factor Receptors
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Nervous System/embryology*
  • Proto-Oncogene Proteins/chemistry
  • Proto-Oncogene Proteins/genetics*
  • Proto-Oncogene Proteins c-ret
  • Receptor Protein-Tyrosine Kinases/chemistry
  • Receptor Protein-Tyrosine Kinases/genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins
PubMed
14660438 Full text @ Development
Abstract
Components of the zebrafish GDNF receptor complex are expressed very early in the development of enteric nervous system precursors, and are already present as these cells begin to enter the gut and migrate caudally along its length. Both gfra1a and gfra1b as well as ret are expressed at this time, while gfra2 expression, the receptor component that binds the GDNF-related ligand neurturin, is not detected until the precursors have migrated along the gut. Gfra genes are also expressed in regions of the zebrafish brain and peripheral ganglia, expression domains conserved with other species. Enteric neurons are eliminated after injection with antisense morpholino oligonucleotides against ret or against both Gfra1 orthologs, but are not affected by antisense oligonucleotides against gfra2. Blocking GDNF signaling prevents migration of enteric neuron precursors, which remain positioned at the anterior end of the gut. Phenotypes induced by injection of antisense morpholinos against both Gfra orthologs can be rescued by introduction of mRNA for gfra1a or for gfra2, suggesting that GFRalpha1 and GFRalpha2 are functionally equivalent.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes