PUBLICATION
MafT, a new member of the small Maf protein family in zebrafish
- Authors
- Takagi, Y., Kobayashi, M., Li, L., Suzuki, T., Nishikawa, K, and Yamamoto, M.
- ID
- ZDB-PUB-040623-3
- Date
- 2004
- Source
- Biochemical and Biophysical Research Communications 320(1): 62-69 (Journal)
- Registered Authors
- Kobayashi, Makoto, Li, Li, Nakajima-Takagi, Yaeko, Nishikawa, Keizo, Suzuki, Takafumi, Yamamoto, Masayuki
- Keywords
- Diethylmaleate, Heterodimer, Luciferase assay, Nrf2, p45, Nfe2, Phase 2 induction, Radiation hybrid mapping, Small Maf protein, Transcription, Zebrafish
- MeSH Terms
-
- Molecular Sequence Data
- Trans-Activators/metabolism
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Cells, Cultured
- Transcription Factors/chemistry*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish
- Sequence Analysis, Protein*
- Amino Acid Sequence
- Animals
- NF-E2-Related Factor 2
- Conserved Sequence
- Phylogeny
- Sequence Homology, Amino Acid
- DNA-Binding Proteins/metabolism
- Gene Expression Regulation, Enzymologic/physiology*
- Maf Transcription Factors, Small
- PubMed
- 15207702 Full text @ Biochem. Biophys. Res. Commun.
Citation
Takagi, Y., Kobayashi, M., Li, L., Suzuki, T., Nishikawa, K, and Yamamoto, M. (2004) MafT, a new member of the small Maf protein family in zebrafish. Biochemical and Biophysical Research Communications. 320(1):62-69.
Abstract
Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. To date, three small Maf proteins, MafF, MafG, and MafK, have been reported in vertebrates, which share redundant functions. In this study, we tried to identify and characterize small Maf proteins in zebrafish to elucidate their conservation and diversity in the fish kingdom. We identified homolog genes of MafG and MafK but not MafF in zebrafish, indicating the former two are conserved among vertebrates. In addition, a novel type of small Maf protein MafT was identified. MafT protein bound MARE sequence as a homodimer or heterodimers with zebrafish Nrf2 or p45 Nfe2. Co-overexpression of MafT and Nrf2 synergistically activated MARE-mediated gene expression in zebrafish embryos. These results indicated that MafT is a new member of small Maf proteins and involved in the Nrf2-dependent gene regulation in cellular defense system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping