PUBLICATION

MafT, a new member of the small Maf protein family in zebrafish

Authors
Takagi, Y., Kobayashi, M., Li, L., Suzuki, T., Nishikawa, K, and Yamamoto, M.
ID
ZDB-PUB-040623-3
Date
2004
Source
Biochemical and Biophysical Research Communications   320(1): 62-69 (Journal)
Registered Authors
Kobayashi, Makoto, Li, Li, Nakajima-Takagi, Yaeko, Nishikawa, Keizo, Suzuki, Takafumi, Yamamoto, Masayuki
Keywords
Diethylmaleate, Heterodimer, Luciferase assay, Nrf2, p45, Nfe2, Phase 2 induction, Radiation hybrid mapping, Small Maf protein, Transcription, Zebrafish
MeSH Terms
  • Trans-Activators/metabolism
  • NF-E2-Related Factor 2
  • Phylogeny
  • Zebrafish
  • Sequence Homology, Amino Acid
  • Sequence Analysis, Protein*
  • Maf Transcription Factors, Small
  • Cells, Cultured
  • Transcription Factors/chemistry*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish Proteins/chemistry*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals
  • DNA-Binding Proteins/metabolism
  • Gene Expression Regulation, Enzymologic/physiology*
  • Conserved Sequence
  • Amino Acid Sequence
  • Molecular Sequence Data
(all 20)
PubMed
15207702 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. To date, three small Maf proteins, MafF, MafG, and MafK, have been reported in vertebrates, which share redundant functions. In this study, we tried to identify and characterize small Maf proteins in zebrafish to elucidate their conservation and diversity in the fish kingdom. We identified homolog genes of MafG and MafK but not MafF in zebrafish, indicating the former two are conserved among vertebrates. In addition, a novel type of small Maf protein MafT was identified. MafT protein bound MARE sequence as a homodimer or heterodimers with zebrafish Nrf2 or p45 Nfe2. Co-overexpression of MafT and Nrf2 synergistically activated MARE-mediated gene expression in zebrafish embryos. These results indicated that MafT is a new member of small Maf proteins and involved in the Nrf2-dependent gene regulation in cellular defense system.
Genes / Markers
Figures
No images available
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
eef1a1l1WTstandard conditions5-9 somitesRTPCR
eef1a1l1WTstandard conditionsDay 4RTPCR
eef1a1l1WTstandard conditionsAdultRTPCR
eef1a1l1WTstandard conditionsDays 7-13RTPCR
eef1a1l1WTstandard conditionsLong-pecRTPCR
eef1a1l1WTstandard conditionsPrim-5RTPCR
eef1a1l1WTstandard conditions75%-epibolyRTPCR
maffWTstandard conditionsLong-pecRTPCR
maffWTstandard conditions5-9 somitesRTPCR
maffWTstandard conditionsDay 4RTPCR
1 - 10 of 35
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Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Takagi, Y., Kobayashi, M., Li, L., Suzuki, T., Nishikawa, K, and Yamamoto, M. (2004) MafT, a new member of the small Maf protein family in zebrafish. Biochemical and Biophysical Research Communications. 320(1):62-69.