PUBLICATION
Organization of cardiac chamber progenitors in the zebrafish blastula
- Authors
- Keegan, B.R., Meyer, D., and Yelon, D.
- ID
- ZDB-PUB-040604-2
- Date
- 2004
- Source
- Development (Cambridge, England) 131(13): 3081-3091 (Journal)
- Registered Authors
- Keegan, Brian, Yelon, Deborah
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastomeres/metabolism
- Blastula/metabolism*
- Cell Lineage
- Endocardium/metabolism
- Fluorescein/pharmacology
- Heart/embryology*
- Heart Ventricles/metabolism
- In Situ Hybridization
- Myocardium/metabolism*
- Nodal Protein
- RNA/metabolism
- Signal Transduction
- Stem Cells/metabolism*
- Time Factors
- Transforming Growth Factor beta/metabolism
- Zebrafish
- PubMed
- 15175246 Full text @ Development
Citation
Keegan, B.R., Meyer, D., and Yelon, D. (2004) Organization of cardiac chamber progenitors in the zebrafish blastula. Development (Cambridge, England). 131(13):3081-3091.
Abstract
Organogenesis requires the specification of a variety of cell types and the organization of these cells into a particular three-dimensional configuration. The embryonic vertebrate heart is organized into two major chambers, the ventricle and atrium, each consisting of two tissue layers, the myocardium and endocardium. The cellular and molecular mechanisms responsible for the separation of ventricular and atrial lineages are not well understood. To test models of cardiac chamber specification, we generated a high-resolution fate map of cardiac chamber progenitors in the zebrafish embryo at 40% epiboly, a stage prior to the initiation of gastrulation. Our map reveals a distinct spatial organization of myocardial progenitors: ventricular myocardial progenitors are positioned closer to the margin and to the dorsal midline than are atrial myocardial progenitors. By contrast, ventricular and atrial endocardial progenitors are not spatially organized at this stage. The relative orientations of ventricular and atrial myocardial progenitors before and after gastrulation suggest orderly movements of these populations. Furthermore, the initial positions of myocardial progenitors at 40% epiboly indicate that signals residing at the embryonic margin could influence chamber fate assignment. Indeed, via fate mapping, we demonstrate that Nodal signaling promotes ventricular fate specification near the margin, thereby playing an important early role during myocardial patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping