PUBLICATION
Multiple Tissue Transformation in Adult Zebrafish by Gene Gun Bombardment and Muscular Injection of Naked DNA
- Authors
- Mudumana, S.P., Low, S., Kwang, J., and Gong, Z.
- ID
- ZDB-PUB-040510-7
- Date
- 2001
- Source
- Marine biotechnology (New York, N.Y.) 3(2): 119-125 (Journal)
- Registered Authors
- Gong, Zhiyuan, Mudumana, Sudha Puttur
- Keywords
- biolistic, DNA vaccine, gene promoter, green fluorescent protein (GFP), muscle
- MeSH Terms
- none
- PubMed
- 14961374 Full text @ Mar. Biotechnol.
Citation
Mudumana, S.P., Low, S., Kwang, J., and Gong, Z. (2001) Multiple Tissue Transformation in Adult Zebrafish by Gene Gun Bombardment and Muscular Injection of Naked DNA. Marine biotechnology (New York, N.Y.). 3(2):119-125.
Abstract
The efficiency of two direct gene transfer methods, gene gun (or particle bombardment) and intramuscular injection, in transforming adult zebrafish tissues in vivo was examined by a noninvasive approach using green fluorescent protein (GFP) reporter gene driven by the ubiquitously expressed human cytomegalovirus promoter. Particle bombardment of adult zebrafish caused internalization and expression of the plasmid only in the superficial layer such as epithelial cells, pigment cells, endothelial cells, and neurons, whereas direct injection primarily transformed muscle fibers of several bundles near or around the injection site. Expression was also evident in several nonmuscle tissues, such as skin epithelia, pigment cells, blood vessel cells, and neuron-like cells. GFP expression persisted for more than 50 days with both methods. These observations indicate the potential of these methods for functional analysis of tissue-specific promoters, delivery of DNA vaccine, and muscular expression of other useful genes.
Errata / Notes
NOTE: First author name in journal and in PubMed is "Sudha, PM" in error.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping