ZFIN ID: ZDB-PUB-040510-1
Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos
Hwang, G., Müller, F., Rahman, M.A., Williams, D.W., Murdock, P.J., Pasi, K.J., Goldspink, G., Farahmand, H., and Maclean, N.
Date: 2004
Source: Marine biotechnology (New York, N.Y.)   6(5): 485-492 (Journal)
Registered Authors: Müller, Ferenc, Williams, Darren W.
Keywords: human coagulation factor VII, transgenic fish, bioreactor
MeSH Terms:
  • Animals
  • Bioreactors*
  • Blood Coagulation/drug effects
  • Cytomegalovirus/genetics
  • DNA, Complementary/genetics
  • Enzyme-Linked Immunosorbent Assay
  • Factor VII/genetics
  • Factor VII/metabolism*
  • Factor VII/pharmacology
  • Fishes/metabolism*
  • Genetic Vectors/genetics
  • Humans
  • Microinjections
  • Photometry
  • Promoter Regions, Genetic/genetics
  • Transgenes/genetics*
  • Zygote/metabolism*
PubMed: 15129328 Full text @ Mar. Biotechnol.
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.