PUBLICATION

Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos

Authors
Hwang, G., Müller, F., Rahman, M.A., Williams, D.W., Murdock, P.J., Pasi, K.J., Goldspink, G., Farahmand, H., and Maclean, N.
ID
ZDB-PUB-040510-1
Date
2004
Source
Marine biotechnology (New York, N.Y.)   6(5): 485-492 (Journal)
Registered Authors
Müller, Ferenc, Williams, Darren W.
Keywords
human coagulation factor VII, transgenic fish, bioreactor
MeSH Terms
  • Animals
  • Bioreactors*
  • Blood Coagulation/drug effects
  • Cytomegalovirus/genetics
  • DNA, Complementary/genetics
  • Enzyme-Linked Immunosorbent Assay
  • Factor VII/genetics
  • Factor VII/metabolism*
  • Factor VII/pharmacology
  • Fishes/metabolism*
  • Genetic Vectors/genetics
  • Humans
  • Microinjections
  • Photometry
  • Promoter Regions, Genetic/genetics
  • Transgenes/genetics*
  • Zygote/metabolism*
PubMed
15129328 Full text @ Mar. Biotechnol.
Abstract
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping