PUBLICATION
Expression analyses of zebrafish transferrin, ifabp, and elastaseB mRNAs as differentiation markers for the three major endodermal organs: Liver, intestine, and exocrine pancreas
- Authors
- Mudumana, S.P., Wan, H., Singh, M., Korzh, V., and Gong, Z.
- ID
- ZDB-PUB-040427-1
- Date
- 2004
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 230(1): 165-173 (Journal)
- Registered Authors
- Gong, Zhiyuan, Korzh, Vladimir, Mudumana, Sudha Puttur, Wan, Haiyan
- Keywords
- fatty acid binding protein, ferric iron, metabolism, yolk, yolk sac, yolk syncytial layer (YSL)
- MeSH Terms
-
- Cell Nucleus/metabolism
- Time Factors
- Intestines/metabolism
- Amino Acid Sequence
- Cell Differentiation
- Sequence Homology, Amino Acid
- Animals
- Carrier Proteins/biosynthesis*
- Carrier Proteins/genetics
- Carrier Proteins/metabolism
- Fatty Acid-Binding Proteins
- Iron/metabolism
- Sequence Analysis, DNA
- DNA, Complementary/metabolism
- Transferrin/biosynthesis*
- Transferrin/genetics
- Liver/metabolism
- Gene Expression Regulation, Developmental*
- Carps
- Molecular Sequence Data
- Blotting, Northern
- RNA, Messenger/metabolism
- In Situ Hybridization
- Pancreas/metabolism
- Serine Endopeptidases/biosynthesis*
- Serine Endopeptidases/genetics
- Zebrafish
- Yolk Sac/metabolism
- PubMed
- 15108321 Full text @ Dev. Dyn.
Citation
Mudumana, S.P., Wan, H., Singh, M., Korzh, V., and Gong, Z. (2004) Expression analyses of zebrafish transferrin, ifabp, and elastaseB mRNAs as differentiation markers for the three major endodermal organs: Liver, intestine, and exocrine pancreas. Developmental Dynamics : an official publication of the American Association of Anatomists. 230(1):165-173.
Abstract
n the present work, three zebrafish cDNA clones encoding transferrin, intestinal fatty acid binding protein (IFABP), and elastaseB were cloned and their expression patterns in early zebrafish development were characterized as differentiation markers for the three major endoderm organs: liver, intestine, and exocrine pancreas. transferrin and ifabp mRNAs exhibit a biphasic expression pattern during early development. transferrin mRNAs were first expressed at approximately 7 hours postfertilization (hpf) in the yolk syncytial layer (YSL) and later in the liver rudiment (from approximately 48 hpf) and in the esophagus transiently (72-96 hpf). Ifabp mRNAs were initially expressed in the YSL at the ventral side during late epiboly (8-9 hpf), spread throughout the YSL of later stage embryos, and appeared in the intestine rudiment at approximately 36 hpf. In contrast to the transferrin and ifabp mRNAs, elastaseB mRNAs were not expressed in the yolk sac or YSL, and these transcripts were detected exclusively in the exocrine pancreas after approximately 56 hpf. Developmental Dynamics 230:165-173, 2004. Copyright 2004 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping