PUBLICATION
Proximal and distal sequences control UV cone pigment gene expression in transgenic zebrafish
- Authors
- Luo, W., Williams, J., Smallwood, P.M., Touchman, J.W., Roman, L.M., and Nathans, J.
- ID
- ZDB-PUB-040225-6
- Date
- 2004
- Source
- The Journal of biological chemistry 279(18): 19286-19293 (Journal)
- Registered Authors
- Roman, Laurie
- Keywords
- none
- MeSH Terms
-
- 5' Untranslated Regions
- Animals
- Animals, Genetically Modified
- Base Sequence
- Gene Expression Regulation*
- Green Fluorescent Proteins
- Luminescent Proteins/genetics
- Molecular Sequence Data
- Promoter Regions, Genetic
- Regulatory Sequences, Nucleic Acid*
- Retinal Cone Photoreceptor Cells/chemistry*
- Rhodopsin/genetics
- Rod Opsins/genetics*
- Ultraviolet Rays
- Zebrafish
- PubMed
- 14966125 Full text @ J. Biol. Chem.
Citation
Luo, W., Williams, J., Smallwood, P.M., Touchman, J.W., Roman, L.M., and Nathans, J. (2004) Proximal and distal sequences control UV cone pigment gene expression in transgenic zebrafish. The Journal of biological chemistry. 279(18):19286-19293.
Abstract
The molecular basis of cone photoreceptor-specific gene expression is largely unknown. In this paper, we define cis-acting DNA sequences that control the cell-type specific expression of the zebrafish ultraviolet (UV) cone pigment gene by transient expression of green fluorescent protein (GFP) transgenes following their injection into zebrafish embryos. These experiments show that 4.8 kb of 5 flanking sequences from the zebrafish UV pigment gene direct expression specifically to UV cones and that this activity requires both distal and proximal sequences. In addition, we demonstrate that a proximal region located between 215 to -110 bp (with respect to the initiator methionine codon) can function in the context of a zebrafish rhodopsin promotor to convert its specificity from rod-only expression to rod and UV cone expression. These experiments demonstrate the power of transient transgenesis in zebrafish to efficiently define cis-acting regulatory sequences in an intact vertebrate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping