PUBLICATION

Sequential antagonism of early and late Wnt-signaling by zebrafish colgate promotes dorsal and anterior fates

Authors
Nambiar, R.M., and Henion, P.D.
ID
ZDB-PUB-040223-1
Date
2004
Source
Developmental Biology   267(1): 165-80 (Journal)
Registered Authors
Henion, Paul, Nambiar, Roopa
Keywords
none
MeSH Terms
  • Animals
  • Cell Lineage*
  • Immunohistochemistry
  • In Situ Hybridization
  • Phenotype
  • Proto-Oncogene Proteins/metabolism*
  • Signal Transduction/physiology*
  • Wnt Proteins
  • Zebrafish/embryology*
  • Zebrafish Proteins*
PubMed
14975724 Full text @ Dev. Biol.
Abstract
The establishment of the vertebrate body plan involves patterning of the ectoderm, mesoderm, and endoderm along the dorsoventral and antero-posterior axes. Interactions among numerous signaling molecules from several multigene families, including Wnts, have been implicated in regulating these processes. Here we provide evidence that the zebrafish colgate(b382) (col) mutation results in increased Wnt signaling that leads to defects in dorsal and anterior development. col mutants display early defects in dorsoventral patterning manifested by a decrease in the expression of dorsal shield-specific markers and ectopic expression of ventrolaterally expressed genes during gastrulation. In addition to these early patterning defects, col mutants display a striking regional posteriorization within the neuroectoderm, resulting in a reduction in anterior fates and an expansion of posterior fates within the forebrain and midbrain-hindbrain regions. We are able to correlate these phenotypes to the overactivation of Wnt signaling in col mutants. The early dorsal and anterior patterning phenotypes of the col mutant embryos are selectively rescued by inactivation of Wnt8 function by morpholino translational interference. In contrast, the regionalized neuroectoderm posterioriorization phenotype is selectively rescued by morpholino-mediated inactivation of Wnt8b. These results suggest that col-mediated antagonism of early and late Wnt-signaling activity during gastrulation is normally required sequentially for both early dorsoventral patterning and the specification and patterning of regional fates within the anterior neuroectoderm.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes