PUBLICATION
Rapid isolation of DNA from fresh and preserved fish scales for polymerase chain reaction
- Authors
- Yue, G.H., and Orban, L.
- ID
- ZDB-PUB-040216-5
- Date
- 2001
- Source
- Marine biotechnology (New York, N.Y.) 3(3): 9-204 (Journal)
- Registered Authors
- Yue, Gen Hua
- Keywords
- Chelex, silica, genotype, marker, microsatellite
- MeSH Terms
- none
- PubMed
- 14961356 Full text @ Mar. Biotechnol.
Citation
Yue, G.H., and Orban, L. (2001) Rapid isolation of DNA from fresh and preserved fish scales for polymerase chain reaction. Marine biotechnology (New York, N.Y.). 3(3):9-204.
Abstract
We developed a simple and inexpensive method to extract DNA from fresh and preserved fish scales. The procedure is based on boiling the scales in 5% Chelex 100, followed by digestion with proteinase K and subsequent absorption of genomic DNA using silica. A single fresh scale from larger species (e.g., tilapia) or a few scales from smaller species (e.g., 4 scales from zebrafish) provide over 200 ng of DNA, enough for at least 40 polymerase chain reaction amplifications. The procedure is applicable for DNA isolation not only from fresh and ethanol-preserved scales, but also from dried and formaldehyde-treated samples, and thus might be useful for investigating specimens stored in museums and other collections. Since the removal of a few scales is a gentle means of sample collection, this technique will allow analysis of genetic diversity, mating systems, and parentage in populations of endangered or ornamental fish with minimal experimental influence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping