PUBLICATION
Systematic identification of factors in zebrafish regulating the early midbrain and cerebellar development by odered differential display and caged mRNA technology
- Authors
- Okamoto, H., Hirate, Y., and Ando, H.
- ID
- ZDB-PUB-040216-15
- Date
- 2004
- Source
- Frontiers in bioscience : a journal and virtual library 9: 93-99 (Journal)
- Registered Authors
- Ando, Hideki, Hirate, Yoshikazu, Okamoto, Hitoshi
- Keywords
- none
- MeSH Terms
-
- Animals
- Biotechnology/methods*
- Cerebellum/embryology
- Cerebellum/physiology*
- Eye Proteins/genetics
- Eye Proteins/physiology*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology*
- LIM-Homeodomain Proteins
- Mesencephalon/embryology
- Mesencephalon/physiology*
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/physiology*
- RNA, Messenger/analysis
- Transcription Factors
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 14766348 Full text @ Front. Biosci.
Citation
Okamoto, H., Hirate, Y., and Ando, H. (2004) Systematic identification of factors in zebrafish regulating the early midbrain and cerebellar development by odered differential display and caged mRNA technology. Frontiers in bioscience : a journal and virtual library. 9:93-99.
Abstract
During brain development, various transcription factors are activated in the regional specific manner and define the identities characteristic to individual regions, and many of such factors have been identified in the vertebrate brain, by taking advantage of the structural and functional conservation of them with the invertebrate counterparts. However, it is still largely unknown why individual transcription factors can define the final morphology and function of the tissues expressing these factors because of the lack of knowledge on which genes are actually up- or down-regulated as downstream targets of individual transcription factors. In this review, we introduce novel technologies which we have invented or improved as a part of our endeavor to identify and functionally analyze the downstream target genes of Isle-3 which are involved in development of the midbrain and the midbrain/hindbrain boundary region in zebrafish embryos. Our strategy and technologies can be applied to analyzing the downstream genes of any other transcription factors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping