ZFIN ID: ZDB-PUB-040204-2
Early requirement for fgf8 function during hindbrain pattern formation in zebrafish
Wiellette, E.L., and Sive, H.
Date: 2004
Source: Developmental dynamics : an official publication of the American Association of Anatomists   229(2): 393-399 (Journal)
Registered Authors: Sive, Hazel, Wiellette, Elizabeth
Keywords: fibroblast growth factor, fgf3, fgf8, zebrafish, hindbrain, valentino, krox20, rhombomere, neural patterning
MeSH Terms:
  • Animals
  • Body Patterning/drug effects
  • Body Patterning/physiology
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Fibroblast Growth Factor 3
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors/antagonists & inhibitors
  • Fibroblast Growth Factors/metabolism*
  • Gene Expression Regulation, Developmental/drug effects
  • In Situ Hybridization
  • Morphogenesis/drug effects
  • Morphogenesis/physiology
  • Oligonucleotides, Antisense/pharmacology
  • Rhombencephalon/embryology*
  • Rhombencephalon/metabolism*
  • Signal Transduction/drug effects
  • Signal Transduction/physiology
  • Somites/drug effects
  • Somites/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
PubMed: 14745965 Full text @ Dev. Dyn.
Fibroblast growth factor (FGF) signaling is required for normal development of the vertebrate brain, including the isthmus and caudal regions of the hindbrain. Recent work in zebrafish has identified a requirement for the combination of fgf3 and fgf8 functions in specification of rhombomeres 5 and 6 (r5, r6), when evaluated at mid- and late somitogenesis stages. However, when examined earlier in development, during early somitogenesis stages, FGF8 alone is required to initiate r5 and r6 development. Both a mutation in fgf8 and injection of fgf8-targeted antisense morpholino-modified oligonucleotides result in suppression of genes normally expressed in r5 and r6 by the one- to two-somite stage. This expression recovers by the six-somite stage, and we propose that this recovery is a response to activation of fgf3 and to delayed accumulation of fgf8. These data demonstrate an early, nonredundant requirement for fgf8 function in hindbrain patterning. Developmental Dynamics 229:393-399, 2004. Copyright 2004 Wiley-Liss, Inc.