PUBLICATION
Proteomics in zebrafish exposed to endocrine disrupting chemicals
- Authors
- Shrader, E.A., Henry, T.R., Greeley Jr., M.S., and Bradley, B.P.
- ID
- ZDB-PUB-040113-9
- Date
- 2003
- Source
- Ecotoxicology (London, England) 12(6): 485-488 (Journal)
- Registered Authors
- Henry, Tala
- Keywords
- proteomics, estradiol, nonylphenol, zebrafish
- MeSH Terms
-
- Animals
- Electrophoresis, Gel, Two-Dimensional
- Embryo, Nonmammalian
- Endocrine System/drug effects
- Estradiol/toxicity*
- Gene Expression Profiling*
- Mutagenicity Tests
- Phenols/toxicity*
- Protein Array Analysis*
- Proteomics*
- Zebrafish/genetics*
- Zebrafish/physiology*
- PubMed
- 14680328 Full text @ Ecotoxicology
Citation
Shrader, E.A., Henry, T.R., Greeley Jr., M.S., and Bradley, B.P. (2003) Proteomics in zebrafish exposed to endocrine disrupting chemicals. Ecotoxicology (London, England). 12(6):485-488.
Abstract
Embryonic zebrafish were examined for changes in protein expression following exposure to sublethal concentrations of 17beta-estradiol (E2) and the estrogen mimic 4-nonylphenol (4-NP). Protein Expression Signatures were derived from embryo homogenates by two-dimensional electrophoresis and digital imaging. In both experiments approximately 30% of the proteins sampled were specific to either E2 or 4-NP and about 33% were common to the control, 4-NP and E2. However, of the proteins induced by either E2 or 4-NP, 28% were common to both chemicals at 1 ppm but only 7% were common to both at 0.1 ppm. While there are many proteins that respond specifically to each chemical, relatively few are common to the two chemicals suggesting that the response pathways of the two chemicals are distinct.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping