Neural cell fate analysis in zebrafish using olig2 BAC transgenics
- Shin, J., Park, H.-C., Topczewska, J.M., Mawdsley, D.J., and Appel, B.
- Methods in cell science : an official journal of the Society for In Vitro Biology 25(1-2): 7-14 (Review)
- Registered Authors
- Appel, Bruce, Mawdsley, David, Park, Hae-Chul, Shin, Jimann, Topczewska, Jolanta
- cerebellum, EGFP, motor neuron, olig2, oligodendrocyte, Purkinje neuron, spinal cord, transgenic, zebrafish
- MeSH Terms
- Cloning, Molecular
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental/physiology*
- Genes, Reporter/genetics
- Genetic Vectors
- Green Fluorescent Proteins
- Luminescent Proteins/metabolism
- Motor Neurons/cytology
- Motor Neurons/metabolism*
- Peripheral Nervous System/cytology
- Peripheral Nervous System/metabolism*
- Purkinje Cells/cytology
- Purkinje Cells/metabolism*
- Zebrafish/anatomy & histology
- 14739582 Full text @ Methods Cell Sci.
Shin, J., Park, H.-C., Topczewska, J.M., Mawdsley, D.J., and Appel, B. (2003) Neural cell fate analysis in zebrafish using olig2 BAC transgenics. Methods in cell science : an official journal of the Society for In Vitro Biology. 25(1-2):7-14.
We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes