ZFIN ID: ZDB-PUB-031204-21
DNA delivery into anterior neural tube of zebrafish embryos by electroporation
Teh, C., Chong, S.W., and Korzh, V.
Date: 2003
Source: Biotechniques   35(5): 950-954 (Journal)
Registered Authors: Chong, Shang Wei, Korzh, Vladimir, Teh, Cathleen
Keywords: none
MeSH Terms:
  • Animals
  • Brain/embryology*
  • Brain/metabolism*
  • DNA/administration & dosage*
  • DNA/genetics
  • DNA/pharmacokinetics*
  • Electrodes
  • Electroporation/instrumentation*
  • Electroporation/methods*
  • Equipment Design
  • Equipment Failure Analysis
  • Gene Transfer Techniques*
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Tissue Distribution
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 14628668
ABSTRACT
The zebrafish is widely used for functional studies of vertebrate genes. It is accessible to manipulations during all stages of embryogenesis because the embryo develops externally and is optically transparent. However, functional studies conducted on the zebrafish have been generally limited to the earliest phase of activity of the gene of interest, which is a limitation in studies of genes that are expressed at various stages of embryonic development. It is therefore necessary to develop methods that allow for the modulation of gene activity during later stages of zebrafish development while leaving earlier functions intact. We have successfully electroporated the green fluorescent protein (GFP) reporter gene into the neural tube of the zebrafish embryo in a unidirectional or bilateral manner. This approach can be used for the functional analysis of the late role of developmental genes in the neural tube of zebrafish embryo and larvae.
ADDITIONAL INFORMATION No data available