PUBLICATION
DNA delivery into anterior neural tube of zebrafish embryos by electroporation
- Authors
- Teh, C., Chong, S.W., and Korzh, V.
- ID
- ZDB-PUB-031204-21
- Date
- 2003
- Source
- Biotechniques 35(5): 950-954 (Journal)
- Registered Authors
- Chong, Shang Wei, Korzh, Vladimir, Teh, Cathleen
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/embryology*
- Brain/metabolism*
- DNA/administration & dosage*
- DNA/genetics
- DNA/pharmacokinetics*
- Electrodes
- Electroporation/instrumentation*
- Electroporation/methods*
- Equipment Design
- Equipment Failure Analysis
- Gene Transfer Techniques*
- Green Fluorescent Proteins
- Luminescent Proteins
- Tissue Distribution
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 14628668 Full text @ Biotechniques
Citation
Teh, C., Chong, S.W., and Korzh, V. (2003) DNA delivery into anterior neural tube of zebrafish embryos by electroporation. Biotechniques. 35(5):950-954.
Abstract
The zebrafish is widely used for functional studies of vertebrate genes. It is accessible to manipulations during all stages of embryogenesis because the embryo develops externally and is optically transparent. However, functional studies conducted on the zebrafish have been generally limited to the earliest phase of activity of the gene of interest, which is a limitation in studies of genes that are expressed at various stages of embryonic development. It is therefore necessary to develop methods that allow for the modulation of gene activity during later stages of zebrafish development while leaving earlier functions intact. We have successfully electroporated the green fluorescent protein (GFP) reporter gene into the neural tube of the zebrafish embryo in a unidirectional or bilateral manner. This approach can be used for the functional analysis of the late role of developmental genes in the neural tube of zebrafish embryo and larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping