PUBLICATION
Syndecan-2 is essential for angiogenic sprouting during zebrafish development
- Authors
- Chen, E., Hermanson, S., and Ekker, S.C.
- ID
- ZDB-PUB-031103-3
- Date
- 2004
- Source
- Blood 103(5): 1710-1719 (Journal)
- Registered Authors
- Ekker, Stephen C., Hermanson, Spencer
- Keywords
- none
- MeSH Terms
-
- 5' Untranslated Regions
- Amino Acid Sequence
- Animals
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- Cytoplasm/metabolism
- Gene Expression Regulation, Developmental*
- Green Fluorescent Proteins
- Heparan Sulfate Proteoglycans/chemistry
- Humans
- In Situ Hybridization
- Luminescent Proteins/metabolism
- Membrane Glycoproteins/biosynthesis*
- Membrane Glycoproteins/physiology*
- Mice
- Molecular Sequence Data
- Neovascularization, Physiologic*
- Phenotype
- Phylogeny
- Protein Binding
- Proteoglycans/biosynthesis*
- Proteoglycans/physiology*
- RNA, Messenger/metabolism
- Signal Transduction
- Syndecan-2
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish
- Zebrafish Proteins
- PubMed
- 14592839 Full text @ Blood
Citation
Chen, E., Hermanson, S., and Ekker, S.C. (2004) Syndecan-2 is essential for angiogenic sprouting during zebrafish development. Blood. 103(5):1710-1719.
Abstract
We used a morpholino-based gene targeting screen to identify a novel protein essential for vascular development using the zebrafish, Danio rerio. We show that syndecan-2, a cell-surface heparan sulfate proteoglycan, is essential for angiogenic sprouting during embryogenesis. The vascular function of syndecan-2 is likely conserved, as zebrafish and mouse syndecan-2 show similar expression patterns around major trunk vessels, and human syndecan-2 can restore angiogenic sprouting in syndecan-2 morphants. In contrast, forced expression of a truncated form of syndecan-2 results in embryos with defects in angiogenesis, indicating the highly-conserved cytoplasmic tail is important for the vascular function of syndecan-2. We further show that VEGF and syndecan-2 genetically interact in vivo using both gain of function and loss of function studies in zebrafish. VEGF-mediated ectopic signaling is compromised in syndecan-2 morphants, and ectopic syndecan-2 potentiates ectopic VEGF signaling. Syndecan-2 as a novel angiogenic factor is a potential candidate for use in the development of angiogenesis-based therapies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping