PUBLICATION
Identification of teleost Thy-1 and association with the microdomain/lipid raft reggie proteins in regenerating CNS axons
- Authors
- Deininger, S.O., Rajendran, L., Lottspeich, F., Przybylski, M., Illges, H., Stürmer, C.A., and Reuter, A.
- ID
- ZDB-PUB-031003-2
- Date
- 2003
- Source
- Molecular and cellular neurosciences 22(4): 544-54 (Journal)
- Registered Authors
- Stuermer, Claudia
- Keywords
- none
- MeSH Terms
-
- Animals
- Axons/metabolism*
- Axons/ultrastructure
- Cells, Cultured
- Central Nervous System/cytology
- Central Nervous System/metabolism*
- DNA, Complementary/analysis
- DNA, Complementary/genetics
- Fibroblasts/cytology
- Fibroblasts/metabolism
- Fish Proteins/metabolism*
- Goldfish/anatomy & histology
- Goldfish/metabolism*
- Growth Cones/metabolism
- Membrane Microdomains/metabolism*
- Membrane Proteins/metabolism*
- Molecular Sequence Data
- Nerve Regeneration/physiology*
- Nerve Tissue Proteins/metabolism*
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/metabolism
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Thy-1 Antigens/genetics
- Thy-1 Antigens/isolation & purification*
- PubMed
- 12727449 Full text @ Mol. Cell Neurosci.
Citation
Deininger, S.O., Rajendran, L., Lottspeich, F., Przybylski, M., Illges, H., Stürmer, C.A., and Reuter, A. (2003) Identification of teleost Thy-1 and association with the microdomain/lipid raft reggie proteins in regenerating CNS axons. Molecular and cellular neurosciences. 22(4):544-54.
Abstract
During regeneration, retinal ganglion cell axons in fish upregulate a cell surface protein that is recognized by the monoclonal antibody (mAB) M802. M802 antigen appeared to be linked to the intracellular, membrane-associated lipid raft/microdomain proteins reggie-1 and reggie-2 that were previously shown to be reexpressed in axon-regenerating neurons [Development 124 (1997), 577]. Here, we report the isolation of the M802 antigen and its identification as the teleost homolog of mammalian Thy-1. Fish Thy-1 is detected in the same detergent-insoluble lipid raft fractions from a fibroblast cell line and from axon regenerating retinae as reggie-1 and 2. Importantly, mAB M802 coimmunoprecipitates reggie-1 and 2 from this lipid raft fraction, implying that fish Thy-1 and reggies interact. This correlates with their colocalization in growing cell processes after M802 antigen/Thy-1 activation with mAB M802. These findings suggest a role of clustered M802 antigen/Thy-1 in reggie raft microdomains for cell growth and axon regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping