PUBLICATION

Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase alpha1 subunit gene (atp1a1a.5)

Authors
Blasiole, B., Canfield, V., Degrave, A., Thisse, C., Thisse, B., Rajarao, J., and Levenson, R.
ID
ZDB-PUB-031001-4
Date
2002
Source
Mechanisms of Development   (Suppl.) 119: S211-S214 (Journal)
Registered Authors
Blasiole, Brian, Canfield, Victor, Degrave, Agnes, Levenson, Robert, Rajarao, S. Johannes, Thisse, Bernard, Thisse, Christine
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • In Situ Hybridization
  • Molecular Sequence Data
  • Sodium-Potassium-Exchanging ATPase*
  • Zebrafish*/genetics
PubMed
14516687 Full text @ Mech. Dev.
Abstract
We have identified and characterized cDNAs encoding a novel zebrafish Na,K-ATPase alpha subunit. The full-length cDNA encodes a 1023-amino-acid-long peptide which shows greatest homology to zebrafish alpha1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na,K-ATPase alpha1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes