PUBLICATION
Facilitated forward chemical genetics using a tagged triazine library and zebrafish embryo screening
- Authors
- Khersonsky, S.M., Jung, D.W., Kang, T.W., Walsh, D.P., Moon, H.S., Jo, H., Jacobson, E.M., Shetty, V., Neubert, T.A., and Chang, Y.T.
- ID
- ZDB-PUB-031001-10
- Date
- 2003
- Source
- Journal of the American Chemical Society 125(39): 11804-11805 (Journal)
- Registered Authors
- Jacobson, Eric, Jo, Hakryul
- Keywords
- none
- MeSH Terms
-
- Animals
- Combinatorial Chemistry Techniques/methods
- Drug Evaluation, Preclinical/methods*
- Triazines/chemistry*
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 14505387 Full text @ J. Am. Chem. Soc.
Citation
Khersonsky, S.M., Jung, D.W., Kang, T.W., Walsh, D.P., Moon, H.S., Jo, H., Jacobson, E.M., Shetty, V., Neubert, T.A., and Chang, Y.T. (2003) Facilitated forward chemical genetics using a tagged triazine library and zebrafish embryo screening. Journal of the American Chemical Society. 125(39):11804-11805.
Abstract
An improved forward chemical genetics approach was successfully demonstrated using a tagged library concept. A small-molecule triazine library with linkers was used to screen for brain/eye developmental phenotypes in a zebrafish embryo system. This approach enabled the rapid isolation of the target proteins by facile affinity matrix preparation and elucidated the first small-molecule inhibitors for several ribosomal accessory proteins or their complex as the target.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping