PUBLICATION
Retinal expression of zebrafish six3.1 and its regulation by Pax6
- Authors
- Wargelius, A., Seo, H.-C., Austobo, L., and Fjose, A.
- ID
- ZDB-PUB-030908-12
- Date
- 2003
- Source
- Biochemical and Biophysical Research Communications 309(2): 475-481 (Journal)
- Registered Authors
- Fjose, Anders, Seo, Hee-Chan
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Eye Proteins
- Gene Expression Regulation, Developmental/drug effects*
- Genes, Regulator/drug effects
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism*
- Homeodomain Proteins/pharmacology*
- Molecular Sequence Data
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/metabolism*
- Paired Box Transcription Factors
- Repressor Proteins
- Retina/embryology*
- Retina/metabolism*
- Sequence Alignment
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 12951074 Full text @ Biochem. Biophys. Res. Commun.
Citation
Wargelius, A., Seo, H.-C., Austobo, L., and Fjose, A. (2003) Retinal expression of zebrafish six3.1 and its regulation by Pax6. Biochemical and Biophysical Research Communications. 309(2):475-481.
Abstract
Homologues of the homeobox genes sine oculis (so) and eyeless (ey) are important regulators of eye development in both vertebrates and invertebrates. A Drosophila paralogue of so, optix, is an orthologue of the vertebrate Six3 gene family. Our analysis of zebrafish six3.1 demonstrated retinal expression in two separate cell layers and the ciliary marginal zone. This pattern is consistent with the observations of Six3 in other vertebrates and indicates functional conservation. We studied the 5(') flanking region of six3.1 and showed that separate enhancing elements are required for expression at different stages of eye development. This analysis also revealed specific binding of zebrafish Pax6.1 protein to an element required for six3.1 expression in ganglion cells. Furthermore, an enhancement of six3.1 transcription by Pax6.1 was observed by co-injection experiments. These results provide evidence for a direct regulatory interaction between vertebrate Pax6 and Six3 genes in eye development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping