PUBLICATION
Comparison of vitellogenin responses in zebrafish and rainbow trout following exposure to environmental estrogens
- Authors
- van den Belt, K., Verheyen, R., and Witters, H.
- ID
- ZDB-PUB-030826-9
- Date
- 2003
- Source
- Ecotoxicology and environmental safety 56(2): 271-281 (Journal)
- Registered Authors
- Witters, Hilda
- Keywords
- none
- MeSH Terms
-
- Animals
- Environmental Exposure*
- Estrogens/toxicity*
- Ethinyl Estradiol/toxicity*
- Male
- Oncorhynchus mykiss/physiology*
- Phenols/toxicity*
- Surface-Active Agents/toxicity*
- Vitellogenins/biosynthesis*
- Zebrafish/physiology*
- PubMed
- 12927559 Full text @ Ecotoxicol. Environ. Saf.
Citation
van den Belt, K., Verheyen, R., and Witters, H. (2003) Comparison of vitellogenin responses in zebrafish and rainbow trout following exposure to environmental estrogens. Ecotoxicology and environmental safety. 56(2):271-281.
Abstract
In this comparative study, the suitability of the commonly used in vivo biomarker for estrogenicity, vitellogenin (VTG), upon waterborne exposure to known environmental estrogens is evaluated in both male zebrafish (Danio rerio) and juvenile rainbow trout (Oncorhynchus mykiss). The results from initial experiments in which both species were exposed to 4-tert-octylphenol (OP) or 17alpha-ethynylestradiol under semistatic conditions for 3 weeks demonstrated a difference in species sensitivity for OP exposure. Additional dose-response studies (semistatic, 3 weeks) with 4-nonylphenol (20, 100, and 500microg/L), bisphenol A (40, 200, and 1000microg/L), dibutylphthalate (40, 200, and 1000microg/L DBP), and 17beta-estradiol (20 and 100ng/L E2) were conducted. All these compounds, except for DBP, were found to be estrogenic to both fish species. The results demonstrated a difference in species sensitivity for NP with the zebrafish being about 5 times less sensitive. For the other compounds tested, no indications for a difference in species sensitivity was found. The results from this study demonstrated that both fish species can be used for the detection of VTG as biomarker for estrogenicity, taken into the potential interspecies differences in sensitivity which might be important for the evaluation of fish population effects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping