ZFIN ID: ZDB-PUB-030826-22
Molecular and functional characterisation of the zebrafish (Danio rerio) PEPT1-type peptide transporter
Verri, T., Kottra, G., Romano, A., Tiso, N., Peric, M., Maffia, M., Boll, M., Argenton, F., Daniel, H., and Storelli, C.
Date: 2003
Source: FEBS letters   549(1-3): 115-122 (Journal)
Registered Authors: Argenton, Francesco, Tiso, Natascia
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Carrier Proteins/physiology*
  • Electrophysiology
  • Embryonic and Fetal Development
  • Hydrogen-Ion Concentration
  • Intestines/metabolism
  • Kinetics
  • Molecular Sequence Data
  • Oocytes
  • Phylogeny
  • Symporters*
  • Tissue Distribution
  • Xenopus
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 12914936 Full text @ FEBS Lett.
We report the molecular and functional characterisation of a novel peptide transporter from zebrafish, orthologue to mammalian and avian PEPT1. Zebrafish PEPT1 is a low-affinity/high-capacity system. However, in contrast to higher vertebrate counterparts in which maximal transport activity is independent of extracellular pH, zebrafish PEPT1 maximal transport rates unexpectedly increase at alkaline extracellular pH. Zebrafish pept1 is highly expressed in the proximal intestine since day 4 post-fertilisation, thus preceding functional maturation of the gut, first feeding and complete yolk resorption. Zebrafish PEPT1 might help to understand the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, zebrafish pept1 can be a useful marker for screening mutations that affect gut regionalisation, differentiation and morphogenesis.