Tenascin-R as a repellent guidance molecule for developing optic axons in zebrafish
- Becker, C.G., Schweitzer, J., Feldner, J., Becker, T., and Schachner, M.
- The Journal of neuroscience : the official journal of the Society for Neuroscience 23(15): 6232-6237 (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas, Feldner, Julia, Schachner, Melitta, Schweitzer, Jörn
- MeSH Terms
- Axons/drug effects
- Cloning, Molecular
- Diencephalon/growth & development
- Fluorescent Dyes
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/physiology
- In Situ Hybridization
- Molecular Probe Techniques
- Molecular Sequence Data
- Nucleic Acid Probes/chemistry
- Nucleic Acid Probes/metabolism
- Nucleic Acid Probes/pharmacology
- Optic Nerve/drug effects
- Optic Nerve/growth & development*
- Optic Nerve/physiology*
- RNA, Messenger/biosynthesis
- Sequence Homology, Amino Acid
- Visual Pathways/cytology
- Visual Pathways/drug effects
- 12867507 Full text @ J. Neurosci.
Becker, C.G., Schweitzer, J., Feldner, J., Becker, T., and Schachner, M. (2003) Tenascin-R as a repellent guidance molecule for developing optic axons in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(15):6232-6237.
To investigate the role of tenascin-R in nervous system development, we studied axon pathfinding in the developing optic system of zebrafish. Zebrafish tenascin-R has the same domain structure as tenascin-R in amniotes. Amino acid sequence identity with human tenascin-R is 60%. In 3-d-old larvae, tenascin-R mRNA is expressed in scattered cells throughout the periventricular cell layer of the diencephalon and tectum. Tenascin-R immunoreactivity is not detectable in the optic nerve, optic tract, or tectal optic neuropil but immediately borders the optic tract caudally. Reducing expression of tenascin-R in 3-d-old larvae in vivo by injecting morpholinos into fertilized eggs led to excessive branching of the optic tract in 86% of all injected larvae compared with 20-37% in controls. Branches were almost exclusively caudal, where tenascin-R immunoreactivity normally borders the optic tract, suggesting a role for tenascin-R in guiding optic axons in the ventral diencephalon by a contact-repellent mechanism.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes