PUBLICATION

phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development

Authors
Lawson, N.D., Mugford, J.W., Diamond, B.A., and Weinstein, B.M.
ID
ZDB-PUB-030717-17
Date
2003
Source
Genes & Development   17(11): 1346-1351 (Journal)
Registered Authors
Diamond, Brianne, Lawson, Nathan, Mugford, Joshua, Weinstein, Brant M.
Keywords
none
MeSH Terms
  • Vascular Endothelial Growth Factor A
  • Endothelial Growth Factors/genetics*
  • Animals, Genetically Modified
  • Animals
  • Vascular Endothelial Growth Factors
  • Gene Expression Regulation, Developmental*
  • Molecular Sequence Data
  • DNA Primers
  • Male
  • Lymphokines/genetics*
  • Muscle, Smooth, Vascular/cytology
  • Muscle, Smooth, Vascular/embryology
  • Phospholipase C gamma
  • Zebrafish/embryology*
  • Polymerase Chain Reaction
  • Base Sequence
  • Genetic Carrier Screening
  • Crosses, Genetic
  • Arteries/cytology
  • Arteries/embryology*
  • Recombinant Proteins/metabolism
  • RNA, Messenger/genetics
  • Intercellular Signaling Peptides and Proteins/genetics*
  • Embryo, Nonmammalian/physiology
  • Female
  • Type C Phospholipases/genetics*
  • Type C Phospholipases/metabolism
PubMed
12782653 Full text @ Genes & Dev.
Abstract
In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find that y10 encodes phospholipase C gamma-1 (plcg1), a known effector of receptor tyrosine kinase signaling. We further show that plcg1y10 mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system.
Genes / Markers
Figures
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping