PUBLICATION
Cloning, pharmacology, and distribution of the neuropeptide Y-receptor Yb in rainbow trout
- Authors
- Larson, E.T., Fredriksson, R., Johansson, S.R.T., and Larhammar, D.
- ID
- ZDB-PUB-030717-14
- Date
- 2003
- Source
- Peptides 24(3): 385-395 (Journal)
- Registered Authors
- Fredriksson, Robert, Larhammar, Dan, Larson, Earl T.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Line
- Cloning, Molecular
- Gene Expression Profiling
- Humans
- Kinetics
- Molecular Sequence Data
- Oncorhynchus mykiss/genetics*
- Phylogeny
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Receptors, Neuropeptide Y/chemistry
- Receptors, Neuropeptide Y/genetics*
- Receptors, Neuropeptide Y/metabolism*
- Sequence Homology, Amino Acid
- PubMed
- 12732336 Full text @ Peptides
Citation
Larson, E.T., Fredriksson, R., Johansson, S.R.T., and Larhammar, D. (2003) Cloning, pharmacology, and distribution of the neuropeptide Y-receptor Yb in rainbow trout. Peptides. 24(3):385-395.
Abstract
This work describes the isolation and pharmacological characterization of a neuropeptide Y (NPY) receptor from rainbow trout (Oncorhynchus mykiss). The receptor exhibits approximately 45% amino acid sequence identity to mammalian Y1-subfamily receptors, Y1, Y4 and y6, a similar degree of identity as these subtypes display to one another. Because it displays highest sequence identity to zebrafish Yb (75%), we named it the trout Yb receptor. The receptor exhibits high binding affinity for zebrafish and human NPY and peptide YY (PYY) but not truncated forms of the peptides. Human pancreatic polypeptide (PP) also binds with high affinity. Y1 selective antagonists exhibit poor binding as is the case for Y2 and Y5 selective ligands. This binding profile supports membership in the Y1 subfamily. Sequence data also support this relationship suggesting that Yb is a fourth and separate member of the Y1 subfamily. NPY has a number of important physiological functions such as regulating food intake and reproduction. The expression of the receptor in the hypothalamus and telencephalon suggests a possible role in these processes. This and other receptors from this species have potential for improving aquaculture.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping