ZFIN ID: ZDB-PUB-030707-5
Representational difference analysis, high-resolution physical mapping, and transcript identification of the zebrafish genomic region for a motor behavior
Sato, T. and Mishina, M.
Date: 2003
Source: Genomics   82(2): 218-229 (Journal)
Registered Authors: Mishina, Masayoshi, Sato, Tomomi
Keywords: Zebrafish; Touch response; Motor behavior mutant; Representational difference analysis;Polymorphic marker; Physical map; SEC14 domain; Synteny
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Blotting, Southern
  • Chromosome Mapping*
  • DNA Primers
  • Gene Components/genetics
  • Genomics/methods*
  • Molecular Sequence Data
  • Movement/physiology*
  • Mutagenesis/genetics
  • Radiation Hybrid Mapping
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Touch/genetics*
  • Zebrafish/genetics*
  • Zebrafish/physiology
PubMed: 12837271 Full text @ Genomics
Zebrafish is one of the best model organisms for investigating gene functions in vertebrates. By 4,5',8-trimethylpsoralen mutagenesis, we isolated a zebrafish mutant, vibrato, with defects in the spontaneous contraction and touch response. Whole genome subtraction between the wild-type and the mutant genomes by representational difference analysis yielded polymorphic markers tightly linked to the vibrato locus. Using these markers, we constructed a high-resolution physical map and localized the vibrato locus within a genomic region of 720 kb. Direct cDNA selection with the contig led to the identification of a novel gene, solo, encoding a protein with SEC14 and spectrin repeat domains. These domains of Solo shared significant amino acid sequence identities with those of mammalian Trio and Karilin. In addition, we found the zebrafish orthologs for mammalian TTN, COL5A2, and CED-6 in the vibrato region. Mapping of these genes localized human chromosomal regions possibly involved in motor disorders. Our results suggest that representational difference analysis provides an efficient way to isolate mutated genomic regions in zebrafish.