PUBLICATION

A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells

Authors
Geldmacher-Voss, B., Reugels, A.M., Pauls, S., and Campos-Ortega, J.A.
ID
ZDB-PUB-030707-11
Date
2003
Source
Development (Cambridge, England)   130(16): 3767-3780 (Journal)
Registered Authors
Campos-Ortega, Jose, Pauls, Stefan, Reugels, Alexander
Keywords
Zebrafish, Neurulation, Mitoses, Orientation, Spindle rotation, Neuroepithelial polarity, ASIP, Histone2A.F/Z:GFP, tau:GFP
MeSH Terms
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Spindle Apparatus/physiology*
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Central Nervous System/cytology
  • Central Nervous System/embryology*
  • Central Nervous System/physiology
  • Biomarkers
  • Epithelial Cells/cytology
  • Epithelial Cells/physiology*
  • Animals
  • Cell Polarity*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Microinjections
  • Transgenes
  • Cell Movement/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Mitosis/physiology*
  • Protein Kinase C/metabolism
  • Recombinant Fusion Proteins/metabolism
  • Isoenzymes
(all 26)
PubMed
12835393 Full text @ Development
Abstract
In the neural plate and neural tube in the trunk region of the zebrafish embryo, dividing cells are oriented parallel to the plane of the neuroepithelium, while in neural keel/rod, cells divide perpendicular to it. This change in the orientation of mitosis is brought about by a 90° rotation of the mitotic spindle. As the two halves of the neural primordium in keel/rod stage are in apposition, the perpendicular orientation of mitoses in this stage determines that daughter cells become allocated to both sides of the neural tube. To assess the role played by cell junctions in controlling the orientation of dividing cells, we studied the expression of components of adherens and tight junctions in the neuroepithelial cells. We find that these proteins are distributed irregularly at the neural plate stage and become polarised apically in the cell membrane only during the keel/rod stage. The stereotypic orientation of mitoses is perturbed only weakly upon loss of function of the cell junction components ASIP and aPKC{lambda}, suggesting that mitotic orientation depends in part on the integrity of cell junctions and the polarity of the epithelium as a whole. However, the 90° rotation of the spindle does not require perfectly polarised cell junctions between the neuroepithelial cells.
Genes / Markers
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Expression
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Phenotype
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca6TgTransgenic Insertion
    kca66TgTransgenic Insertion
      m129
        		Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Fish
        Antibodies
        Orthology
        Engineered Foreign Genes
        No data available
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        Citation
        Geldmacher-Voss, B., Reugels, A.M., Pauls, S., and Campos-Ortega, J.A. (2003) A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells. Development (Cambridge, England). 130(16):3767-3780.