ZFIN ID: ZDB-PUB-030707-10
vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain
Wiellette, E.L. and Sive, H.
Date: 2003
Source: Development (Cambridge, England)   130(16): 3821-3829 (Journal)
Registered Authors: Sive, Hazel, Wiellette, Elizabeth
Keywords: Vhnf1, Fibroblast growth factor, Fgf3, Fgf8, Zebrafish, Hindbrain, Valentino, Krox20, Rhombomere, Neural patterning
MeSH Terms:
  • Animals
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Epistasis, Genetic
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism*
  • Gene Expression Regulation, Developmental*
  • Hepatocyte Nuclear Factor 1-beta
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • In Situ Hybridization
  • MafB Transcription Factor
  • Morphogenesis
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism*
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Rhombencephalon/anatomy & histology
  • Rhombencephalon/embryology*
  • Rhombencephalon/physiology
  • Signal Transduction/physiology*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins*
PubMed: 12835397 Full text @ Development
Vertebrate hindbrain segmentation is a highly conserved process but the mechanism of rhombomere determination is not well understood. Recent work in the zebrafish has shown a requirement for fibroblast growth factor (Fgf) signaling and for the transcription factor variant hepatocyte nuclear factor 1 (vhnf1) in specification of rhombomeres 5 and 6 (r5+r6). We show here that vhnf1 functions in two ways to subdivide the zebrafish caudal hindbrain domain (r4-r7) into individual rhombomeres. First, vhnf1 promotes r5+r6 identity through an obligate synergy with Fgf signals to activate valentino and krox20 expression. Second, vhnf1 functions independently of Fgf signals to repress hoxb1a expression. Although vhnf1 is expressed in a broad posterior domain during gastrulation, it promotes the specification of individual rhombomeres. This is achieved in part because vhnf1 gives cellular competence to respond to Fgf signals in a caudal hindbrain-specific manner.