PUBLICATION
Imaging intercellular calcium waves during late epiboly in intact zebrafish embryos
- Authors
- Webb, S.E. and Miller, A.L.
- ID
- ZDB-PUB-030702-1
- Date
- 2003
- Source
- Zygote (Cambridge, England) 11(2): 175-182 (Journal)
- Registered Authors
- Miller, Andrew L., Webb, Sarah E.
- Keywords
- none
- MeSH Terms
-
- Aequorin/metabolism
- Animals
- Calcium/metabolism*
- Embryo, Nonmammalian/metabolism
- Fluorescent Dyes/metabolism
- Microscopy, Confocal
- Microscopy, Fluorescence
- Organic Chemicals
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 12828417 Full text @ Zygote
Citation
Webb, S.E. and Miller, A.L. (2003) Imaging intercellular calcium waves during late epiboly in intact zebrafish embryos. Zygote (Cambridge, England). 11(2):175-182.
Abstract
Through the injection of f-aequorin and the use of a photon imaging microscope, we have previously reported that a rhythmic series of intercellular Ca2+ waves circumnavigate zebrafish embryos over a 10 h period during gastrulation and axial segmentation. These waves first appear at about 65% epiboly and continue to arise every 5-10 min up to at least the 16-somite stage. In response to our publication, it was suggested that the waves may be an artefact caused by dechorionation of the embryos and would not be observed during the development of intact embryos (i.e. those with chorions). Here we demonstrate (again initially by aequorin imaging) that the rhythmic intercellular Ca2+ waves that traverse the blastoderm margin can also be observed in embryos that have an intact chorion. In addition, the appearance time, propagation pathway, velocity, duration and Ca2+ rise of the waves, as well as the interwave interval and the timing of wave onset, are approximately the same in both dechorionated embryos and those with an intact chorion. Furthermore, by loading intact embryos with Ca(2+)-green dextran at the single-cell stage and then using scanning confocal microscopy to obtain high-resolution images, we confirm the presence of circumferential Ca2+ waves and show that they pass through a population of deep cells located at the blastoderm margin. The confirmation of these pan-embryonic Ca2+ waves in zebrafish further corroborates our earlier suggestion that such waves might play a fundamental role in normal embryonic patterning during the gastrula period.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping