PUBLICATION

SHH and FGF8 play distinct roles during development of noradrenergic neurons in the locus coeruleus of the zebrafish

Authors

Lam, C.S., Sleptsova-Friedrich, I., Munro, A.D., and Korzh, V.

ID

ZDB-PUB-030602-5

Date

2003

Source

Molecular and cellular neurosciences 22(4): 501-515 (Journal)

Registered Authors

Korzh, Vladimir, Lam, Eric (C.S.), Sleptsova-Freidrich, Inna

Keywords

none

MeSH Terms

Protein Synthesis Inhibitors/pharmacology
Down-Regulation/genetics
Apoptosis/genetics
Hedgehog Proteins
Fibroblast Growth Factor 8
Activin Receptors, Type I/genetics
Activin Receptors, Type I/metabolism
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism
Protein Serine-Threonine Kinases*
Locus Coeruleus/cytology
Locus Coeruleus/embryology*
Locus Coeruleus/metabolism
Animals
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/embryology*
Embryo, Nonmammalian/metabolism
Fibroblast Growth Factors/deficiency*
Fibroblast Growth Factors/genetics
Receptors, Growth Factor*
Norepinephrine/biosynthesis*
Gene Expression Regulation, Developmental/genetics
Neurons/cytology
Neurons/metabolism*
Trans-Activators/deficiency*
Trans-Activators/genetics
Cell Survival/genetics
Bone Morphogenetic Protein Receptors, Type I
Protein Biosynthesis/drug effects
Protein Biosynthesis/genetics
Mutation/genetics
Up-Regulation/genetics
Tyrosine 3-Monooxygenase/metabolism

(all 34)

PubMed

12727446 Full text @ Mol. Cell Neurosci.

CTD

12727446

Abstract

Several signaling pathways have been implicated in the development of dopaminergic and serotonergic neurons. Here, we analyzed the formation of noradrenergic (NAergic) cells in the locus coeruleus (LC) of zebrafish. In the sonic hedgehog (shh) mutant, cells positive for tyrosine hydroxylase, a marker for putative NAergic cells in the LC were reduced. Similarly, the inhibition of translation of all hh genes and the perturbation of Shh signaling by forskolin resulted in a decrease in the number of cells. Conversely, when SHH was overexpressed, an increase in number was observed. Thus, Shh is involved in maintaining the appropriate number of cells in the LC. While elevated levels of bone morphogenetic protein 4 (BMP4) did not attenuate tyrosine hydroxylase-positive cells, exogenous fibroblast growth factor 8 (FGF8) rescued NAergic neurons in the acerebellar (ace) mutant, providing direct in vivo evidence that Fgf8 is required for the induction of NAergic neurons in the LC.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
Df(Chr07)t4/t4	standard conditions	Long-pec	locus coeruleus neuron decreased amount, abnormal	Fig. 2
WT + MO3-ihhb + MO3-shha + MO3-shhb	standard conditions	Long-pec	blood circulation disrupted, abnormal	Fig. 2
WT + MO3-ihhb + MO3-shha + MO3-shhb	standard conditions	Long-pec	locus coeruleus neuron decreased amount, abnormal	Fig. 2
WT + MO3-ihhb + MO3-shha + MO3-shhb	standard conditions	Long-pec	pericardium edematous, abnormal	Fig. 2
WT + MO3-ihhb + MO3-shha + MO3-shhb	standard conditions	Long-pec	somite U-shaped, abnormal	Fig. 2
fgf8a^{ti282a/ti282a}	standard conditions	Long-pec	forebrain neuron normal amount, normal	Fig. 6
fgf8a^{ti282a/ti282a}	standard conditions	Long-pec	forebrain neuron spatial pattern, normal	Fig. 6
fgf8a^{ti282a/ti282a}	standard conditions	Long-pec	locus coeruleus neuron decreased amount, abnormal	Fig. 6

1 - 8 of 8

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
t4		Deficiency	shha
ti282a		Point Mutation	fgf8a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
ihhb	MO3-ihhb	MRPHLNO
shha	MO3-shha	MRPHLNO
shhb	MO3-shhb	MRPHLNO

Fish

Fish
Df(Chr07)t4/t4
fgf8a^{ti282a/ti282a}
WT + MO3-ihhb + MO3-shha + MO3-shhb

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Lam et al., 2003 ZDB-PUB-030602-5 PMID:12727446

SHH and FGF8 play distinct roles during development of noradrenergic neurons in the locus coeruleus of the zebrafish

Lam et al., 2003

ZDB-PUB-030602-5
PMID:12727446