|ZFIN ID: ZDB-PUB-030425-2|
The pro-opiomelanocortin gene of the zebrafish (Danio rerio)
Hansen, I.A., To, T.T., Wortmann, S., Burmester, T., Winkler, C., Meyer, S.R., Neurner, C., Fassnacht, and Allolio, B.
|Source:||Biochemical and Biophysical Research Communications 303(4): 1121-1128 (Journal)|
|Registered Authors:||Winkler, Christoph|
|PubMed:||12684052 Full text @ Biochem. Biophys. Res. Commun.|
Hansen, I.A., To, T.T., Wortmann, S., Burmester, T., Winkler, C., Meyer, S.R., Neurner, C., Fassnacht, and Allolio, B. (2003) The pro-opiomelanocortin gene of the zebrafish (Danio rerio). Biochemical and Biophysical Research Communications. 303(4):1121-1128.
ABSTRACTThe cDNA and the gene for pro-opiomelanocortin (POMC) in the zebrafish ( Danio rerio) were isolated and analyzed. The gene consists of three exons and two short introns and has a similar overall structural organization as in Homo sapiens. Intron 1 (339bp) divides the 5(') untranslated region from the coding region while intron 2 (1522bp) is located between the signal peptide and the sequence encoding ACTH. Transcription starts 26bp downstream of a TATA box and there is one polyadenylation signal in the 3(') untranslated region. The cDNA comprises of 964bp with an open reading frame encoding a 222 amino acid hormone prepropeptide that is split into six putative hormones. Sequence comparison of zebrafish POMC to sequences of various other vertebrate species reveals four regions that are highly conserved during the evolution of vertebrates-the N-terminal region, ACTH, beta-MSH, and beta -endorphin, whereas the connecting peptides show a much higher degree of variability. Phylogenetic analysis of the POMC sequences of various vertebrate species resulted in the expected pattern of species evolution In situ hybridization demonstrated POMC expression in a cluster of cells (corticotrophs) in the pituitary of the zebrafish as early as 23h after fertilization. These findings will facilitate the use of the zebrafish as a model organism in the study of the physiological role of POMC-derived peptides.