PUBLICATION
Evidence of a novel transduction pathway mediating detection of polyamines by the zebrafish olfactory system
- Authors
- Michel, W.C., Sanderson, M.J., Olson, J.K., and Lipschitz, D.L.
- ID
- ZDB-PUB-030425-1
- Date
- 2003
- Source
- The Journal of experimental biology 206(10): 1697-1706 (Journal)
- Registered Authors
- Michel, William C.
- Keywords
- none
- MeSH Terms
-
- Adaptation, Physiological
- Animals
- Biogenic Polyamines/analysis*
- Biogenic Polyamines/pharmacology
- Colforsin/pharmacology
- Electrophysiology
- Female
- Male
- Odorants/analysis*
- Olfactory Receptor Neurons/drug effects
- Olfactory Receptor Neurons/physiology
- Second Messenger Systems
- Signal Transduction
- Smell/drug effects
- Smell/physiology*
- Zebrafish/physiology*
- PubMed
- 12682101 Full text @ J. Exp. Biol.
Citation
Michel, W.C., Sanderson, M.J., Olson, J.K., and Lipschitz, D.L. (2003) Evidence of a novel transduction pathway mediating detection of polyamines by the zebrafish olfactory system. The Journal of experimental biology. 206(10):1697-1706.
Abstract
To better understand the full extent of the odorant detection capabilities of fish, we investigated the olfactory sensitivity of zebrafish to a monoamine and several polyamines using electrophysiological and activity-dependent labeling techniques. Electro -olfactogram (EOG) recording methods established the relative stimulatory effectiveness of these odorants as: spermine >> spermidine approximately agmatine > glutamine > putrescine >/= cadaverine >/= histamine > artificial freshwater. The detection threshold for the potent polyamines was approximately 1 micro mol l(-1). Cross-adaptation experiments suggested that multiple receptors are involved in polyamine detection. Three observations indicated that polyamine signaling may involve a transduction cascade distinct from those used by either amino acids or bile salts. Like bile salts and the adenylate cyclase activator forskolin, but unlike amino acid odorants, polyamines failed to stimulate activity-dependent labeling of olfactory sensory neurons with the cation channel permeant probe agmatine, suggesting a signaling pathway different from that used by amino acid stimuli. Also supporting distinct amino acid and polyamine signaling pathways is the finding that altering phospholipase C activity with the inhibitor U-73122 significantly reduced amino acid-evoked responses, but had little effect on polyamine- (or bile salt-) evoked responses. Altering cyclic nucleotide-mediated signaling by adenylate cyclase activation with forskolin, which significantly reduced responses to bile salts, failed to attenuate polyamine responses, suggesting that polyamines and bile salts do not share a common transduction cascade. Collectively, these findings suggest that polyamines are a new class of olfactory stimuli transduced by a receptor-mediated, second messenger signaling pathway that is distinct from those used by amino acids or bile salts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping