ZFIN ID: ZDB-PUB-030219-1
In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina
Das, T., Payer, B., Cayouette, M., and Harris, W.A.
Date: 2003
Source: Neuron   37(4): 597-609 (Journal)
Registered Authors: Harris, William A.
Keywords: none
MeSH Terms:
  • Animals
  • Cell Division/physiology
  • Fluorescent Dyes
  • Luminescent Proteins/genetics
  • Microscopy, Video
  • Mitosis
  • Mutation
  • Neurons/cytology*
  • Rats
  • Recombinant Fusion Proteins/genetics
  • Retina/cytology*
  • Retina/embryology*
  • Stem Cells/cytology
  • Time Factors
  • Zebrafish
PubMed: 12597858 Full text @ Neuron
Asymmetric cell division has been proposed to be a mechanism for generating cell fate diversity. Evidence in both vertebrates and invertebrates has suggested that division of neuroblasts along the apico-basal axis allows for unequal segregation of cell fate determinants and consequently distinct cell fates for the resulting daughter cells. Here, Das et al. used in vivo two-photon imaging to reconstruct cell divisions in the zebrafish retina and show that, surprisingly, there are no apico-basal cell divisions in the zebrafish retina during the period of neurogenesis. Rather, the period of neurogenesis correlates with an unexpected shift in orientation of cell division from central-peripheral to circumferential. Further, in mutants that exhibit delayed timing of neurogenesis and differentiation, this shift is also delayed, suggesting a role for this shift in initiating neurogenesis in the zebrafish retina.