PUBLICATION

Light stimulates a transducin-independent increase of cytoplasmic Ca2+ and suppression of current in cones from the zebrafish mutant nof

Authors
Brockerhoff, S.E., Rieke, F., Matthews, H.R., Taylor, M.R., Kennedy, B., Ankoudinova, I., Niemi, G.A., Tucker, C.L., Xiao, M., Cilluffo, M.C., Fain, G.L., and Hurley, J.B.
ID
ZDB-PUB-030210-3
Date
2003
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   23(2): 470-480 (Journal)
Registered Authors
Brockerhoff, Susan, Hurley, James B., Kennedy, Breandan N., Niemi, Gregory, Taylor, Michael
Keywords
genetic analysis of phototransduction, transducin, cone photoreceptor physiology, light adaptation, photoreceptor mutations, G-protein-mediated signal transduction
MeSH Terms
  • Homozygote
  • Amino Acid Sequence
  • Cytoplasm/metabolism*
  • Light*
  • Adaptation, Ocular/genetics
  • Adaptation, Ocular/physiology
  • Vision, Ocular/genetics
  • Vision, Ocular/physiology
  • Animals
  • Larva
  • Calcium/metabolism*
  • Physical Chromosome Mapping
  • Mutagenesis
  • In Situ Hybridization
  • Signal Transduction/physiology
  • Point Mutation
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger/biosynthesis
  • Zebrafish
  • Transducin/deficiency
  • Transducin/genetics
  • Transducin/metabolism*
  • GTP-Binding Proteins/metabolism
  • Cyclic GMP/metabolism
  • Retinal Cone Photoreceptor Cells/cytology
  • Retinal Cone Photoreceptor Cells/metabolism
  • Retinal Cone Photoreceptor Cells/radiation effects*
(all 28)
PubMed
12533607 Full text @ J. Neurosci.
Abstract
Transducins couple visual pigments to cGMP hydrolysis, the only recognized phototransduction pathway in vertebrate photoreceptors. Here we describe a zebrafish mutant, no optokinetic response f(w21) (nof), with a nonsense mutation in the gene encoding the alpha subunit of cone transducin. Retinal morphology and levels of phototransduction enzymes are normal in nof retinas, but cone transducin is undetectable. Dark current in nof cones is also normal, but it is insensitive to moderate intensity light. The nof cones do respond, however, to bright light. These responses are produced by a light-stimulated, but transducin- independent, release of Ca2+ into the cone cytoplasm. Thus, in addition to stimulating transducin, light also independently induces release of Ca2+ into the photoreceptor cytoplasm.
Genes / Markers
Figures
No images available
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
w21
    		Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    Human Disease Fish Conditions Evidence
    cone-rod dystrophygnat2w21/w21 (AB)standard conditionsTAS
    1 - 1 of 1
    Show
    Sequence Targeting Reagents
    No data available
    Fish
    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Brockerhoff, S.E., Rieke, F., Matthews, H.R., Taylor, M.R., Kennedy, B., Ankoudinova, I., Niemi, G.A., Tucker, C.L., Xiao, M., Cilluffo, M.C., Fain, G.L., and Hurley, J.B. (2003) Light stimulates a transducin-independent increase of cytoplasmic Ca2+ and suppression of current in cones from the zebrafish mutant nof. The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(2):470-480.