ZFIN ID: ZDB-PUB-030116-6
Zinc finger protein too few controls the development of monoaminergic neurons
Levkowitz, G., Zeller, J., Sirotkin, H.I., French, D., Schilbach, S., Hashimoto, H., Hibi, M., Talbot, W.S., and Rosenthal, A.
Date: 2003
Source: Nature Neuroscience   6(1): 28-33 (Journal)
Registered Authors: Hashimoto, Hisashi, Hibi, Masahiko, Levkowitz, Gil, Sirotkin, Howard, Talbot, William S., Zeller, Joerg
Keywords: none
MeSH Terms:
  • Animals
  • Anti-Bacterial Agents/chemical synthesis*
  • Apoptosis/genetics
  • Biogenic Monoamines/metabolism*
  • Brain/cytology
  • Brain/embryology*
  • Brain/metabolism
  • Brain Tissue Transplantation
  • Carrier Proteins/genetics
  • Carrier Proteins/isolation & purification*
  • Cell Differentiation/genetics*
  • Chromosome Mapping
  • Dopamine/metabolism
  • Embryo, Nonmammalian
  • Female
  • Gene Expression Regulation, Developmental/genetics
  • Male
  • Neurons/cytology
  • Neurons/metabolism*
  • Peptides*
  • Repressor Proteins/genetics
  • Repressor Proteins/isolation & purification
  • Serotonin/metabolism
  • Signal Transduction/genetics
  • Stem Cells/cytology
  • Stem Cells/metabolism*
  • Zebrafish
  • Zebrafish Proteins*
  • Zinc Fingers/genetics
PubMed: 12469125 Full text @ Nat. Neurosci.
The mechanism controlling the development of dopaminergic (DA) and serotonergic (5HT) neurons in vertebrates is not well understood. Here we characterized a zebrafish mutant-too few (tof)-that develops hindbrain 5HT and noradrenergic neurons, but does not develop hypothalamic DA and 5HT neurons. tof encodes a forebrain-specific zinc finger transcription repressor that is homologous to the mammalian Fezl (forebrain embryonic zinc finger-like protein). Mosaic and co-staining analyses showed that fezl was not expressed in DA or 5HT neurons and instead controlled development of these neurons non-cell-autonomously. Both the eh1-related repressor motif and the second zinc finger domain were necessary for tof function. Our results indicate that tof/fezl is a key component in regulating the development of monoaminergic neurons in the vertebrate brain.